LOCUS NZ_DS981517 22178 bp DNA linear CON 30-MAR-2017 DEFINITION Clostridium sporogenes ATCC 15579 Scfld_02_0, whole genome shotgun sequence. ACCESSION NZ_DS981517 VERSION NZ_DS981517.1 KEYWORDS . SOURCE Clostridium sporogenes ATCC 15579 ORGANISM Clostridium sporogenes ATCC 15579 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT REFSEQ INFORMATION: The reference sequence was derived from DS981517. Clostridium sporogenes (GenBank Accession Number for 16S rDNA gene: DQ911258*) is a member of the Firmicutes division of the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from ATCC (ATCC 15579). We have collected 8.9X coverage in plasmid end reads and 454 reads. We have performed one round of automated sequence improvement(pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs.These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gutmicrobiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representativesof the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. tRNA genes were determined using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and Rfam v8.0. Gene names are generated at the contig level and may not necessarily reflect any known order or orientation between contigs. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in May 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. Clostridium sporogenes (GenBank Accession Number for 16S rDNA gene: DQ911258*) is a member of the Firmicutes division of the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from ATCC (ATCC 15579). We have collected 8.9X coverage in plasmid end reads and 454 reads. We have performed one round of automated sequence improvement(pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs.These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gutmicrobiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representativesof the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline set; GeneMarkS+ repeat_region ##antiSMASH-Data-START## Version :: 5.0.0alpha Run date :: 2018-12-14 14:15:44 NOTE: This is a single cluster extracted from a larger record! Orig. start :: 962428 Orig. end :: 984606 ##antiSMASH-Data-END## FEATURES Location/Qualifiers CDS 1..1404 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255265.1" /locus_tag="CLOSPO_RS04655" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02256" /product="hypothetical protein" /protein_id="WP_003484318.1" /transl_table=11 /translation="MSNLYEKYKNARVHAPNFPMTIEWIGAKKTNLDKLKNRVIVLDFW TFCCINCIHVIEDLKYLEEKYKNKLQVIGVHSPKFKYEKNSKAILNAMKKYGISHPVVN DKNKSIWDSYTVNAWPTFVLIDPEGYAFAMVSGEGNRELLDQIIGDTIEYFDNIHWSEE NIVLQKKCEKEEYIYPSKISINENGLIAFSEKGRNRIVILDQNLEKIKTIGSSEYGLKD GDFKEAQFKAPEGLRFIENKIYVADTENHSIRECDLEKEMVKTIAGNGQKEYSPMAKGD ALNVSLNSPWDLEILEDCIYIAMAGNHQIWKYNMKDKSIESHIGTGGENIRDGSFDKCL LAQTSALCYDGKKRLYFVDSETSSIRYADLEEHKVHTLIGKGLFYFGSKVGSFENTMLQ HPLDLKYKDNVLYIADTYNNRIIKADILNEKVEIIKKGLNEPNGIAIYEDSIYMCNTNN GKIEKINMK" cluster 1..22178 /aStool="rule-based-clusters" /cluster_number="3" /contig_edge="False" /core_location="[971863:975442](+)" /cutoff="20000" /detection_rule="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /neighbourhood="10000" /parent_cluster_number="3" /product="porA" /tool="antismash" cluster_core 9436..13014 /aStool="rule-based-clusters" /cluster_number="3" /cutoff="20000" /detection_rule="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /neighbourhood="10000" /parent_cluster_number="3" /product="porA" supercluster 1..22178 /child_cluster="3" /contig_edge="False" /detection_rules="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /kind="single" /product="porA" /supercluster_number="3" /tool="antismash" region 1..22178 /contig_edge="False" /product="porA" /rules="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /supercluster_numbers="3" /tool="antismash" gene 1..1404 /locus_tag="CLOSPO_RS04655" /old_locus_tag="CLOSPO_02256" gene complement(1426..2244) /locus_tag="CLOSPO_RS04660" /old_locus_tag="CLOSPO_02257" CDS complement(1426..2244) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255264.1" /locus_tag="CLOSPO_RS04660" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02257" /product="AP endonuclease" /protein_id="WP_042384514.1" /transl_table=11 /translation="MDRLLFGISGLPIGDGTTKFNYKSGITYLNNIGLDAMELPFVRSV NVTDKNKEGILEEKNNSNFYLSAHGSYFINLNADEIEKQEKSLERIIKGAEGLKKVQGR SLIFHPGFYLKDSKEETFKTILENLKKLPDLGVDYRLETTGKGTQFGSLEENVALCKEV ATCKLCIDFSHIHARSNGGLKTYKDFYNILSYVEKNLGRSALDDMHIHLSGIHYSEKGE RNHLPFEESDFNYIACLKAFKDFNINGCIICESPILEKDALLLKNSYYEL" gene 2435..4216 /locus_tag="CLOSPO_RS04665" /old_locus_tag="CLOSPO_02258" CDS 2435..4216 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255263.1" /locus_tag="CLOSPO_RS04665" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02258" /product="single-stranded-DNA-specific exonuclease RecJ" /protein_id="WP_042384515.1" /transl_table=11 /translation="MKKKWMLRRSKLNIKDIAHKSGISETLCTILVNREIYNLEDIESF LNPSLKKLYNPLLMKDMDKGTEIIKKAIIDKKKIAIYGDYDADGVTSTVILYTALKECE ANVTYYIPDRETEGYGMCTDRVEKLKEEGVEVIITCDNGIAALEQVERAKELGMTVVIT DHHELPFIENENGEREYVVPKADVIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNM NPNKYKELLEFAAIGTICDVVDLKDENRIIAKLGLKNINNTNNLGLRALIKETSLNGKN ITSYNVGFIIGPCINATGRLDTAALSVELFLTKDIKRAEELAKELRNLNTERQEITMEG VEEITYTIENSSLKNDKVLVIYKEDIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGS GRSIDGYNLFEELMKCKEYIYKFGGHPMAAGLTIEEKNIDKIREELNKNCNLKDEDFIP KIRIDKRLPLKEVNLELIDSLQCLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLT CRIEDTNRRIDAIAFGKVDEFKRDLENIYGDESIENIITNPLMAGLKLDLIYYPMINEY NGNVYTQLRIIDYRINR" gene 5025..7133 /locus_tag="CLOSPO_RS04670" /old_locus_tag="CLOSPO_02259" CDS 5025..7133 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255262.1" /locus_tag="CLOSPO_RS04670" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02259" /product="PAS domain S-box protein" /protein_id="WP_003484321.1" /transl_table=11 /translation="MGPIGSVFLEDKNTIKSIMDSIPYSCYIVNDKYELLYVNSIALKL SKELKRKSSSKLRPGEEKTLMDCLDEYEINFIDGEPVTEKNFPVFIALKEQKKIKEVSL IFTKNNCEKVCFSLTVIPINLEGTKKGALVNLMDITQEHTYKETIEKDKEEILNISEQI KNKSEIIDILRKKELDYIKYLNNVINNVSEGILVFDKHSKLILWNKAIEQIGGMKIKKI LGIKEIINEYDIYILDNHKNMKYKIKKVNPDYNSSVKDLLLKLVKKDSNEIKYIEYNSN PIIDENNEILYTISSIKNVTDLIESQIISEERAEFIKYVVDCIELPLAVLDYPDLTYKL VNERYEELVERLYTKKIKGNLLGNTVEDVFRKEEFKEQLEVLQKMKEGTIKEYTFSPEK FVLQSGEERFFKVKYIYHETKNKKRVHIHGSDVTEEFKNNLELEKVNKLKDEFFTIISH ELRTPLTIIYSSLQLAYDIYSKDINPSIDKILKRINQNTARLLKLINNILDISKAEAGF LALNNEYFDVVWETENIVNSITNYANSKNINLIFDTTKEEEKVWIDKDKYERILLNLLS NAVKFTPENKSIYVTLKIYKNNFKLIVKDEGAGIPKEKIDCIFNRFAQVNSSLSRNAEG TGIGLSLVKKIVDLMGGEIKVNSTLGKGTTFTVKINKNMDKCYEKIEKTVIHNDISKKI HIEFSDIN" gene complement(7247..7396) /locus_tag="CLOSPO_RS04675" /old_locus_tag="CLOSPO_02260" CDS complement(7247..7396) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255261.1" /locus_tag="CLOSPO_RS04675" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02260" /product="small, acid-soluble spore protein, alpha/beta type" /protein_id="WP_080333629.1" /transl_table=11 /translation="MPEVKEALNRFRIESSKDMEIGSTNFNNPKNFSNKVKLNSDESFN PYKF" gene 7740..8804 /locus_tag="CLOSPO_RS04680" /old_locus_tag="CLOSPO_02261" CDS 7740..8804 /EC_number="2.4.1.227" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255260.1" /locus_tag="CLOSPO_RS04680" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02261" /product="undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" /protein_id="WP_042384517.1" /transl_table=11 /translation="MKKIIMTGGGTAGHVTPNLALVPELKKLGYEIKYIGSIEGIERKI IKKEGIEYFPISSGKLRRYFDLKNFSDPFKVLKGVFQAKKIIKREKPDIIFSKGGFVTV PVVIAAHLNKIPVIAHESDITPGLANKLATPYCTKVCVTFPESIKHIKGDKAVLTGTPI RKELLEGSKVKGIELCGFKDDKPILLIIGGSLGSKIINEIVRKNLDDILLKFNIIHICG KSNLDENLENRKGYAQFEYVNEELPDLMKASDLVISRAGANVIYELLALKKPNLLIPLS KKSSRGDQILNAGSFEKSGYSLVLKEEELSDKTLMEKLSYLYENRNVYINNMSKSKMDN GVKNITELIKKYTK" gene 9436..13014 /locus_tag="CLOSPO_RS04685" /old_locus_tag="CLOSPO_02262" CDS 9436..13014 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) porA: POR_N" /gene_functions="biosynthetic (rule-based-clusters) porA: Fer4" /gene_functions="biosynthetic (rule-based-clusters) porA: TPP_enzyme_C" /gene_functions="biosynthetic (rule-based-clusters) porA: POR" /gene_functions="biosynthetic (rule-based-clusters) porA: Fer4_10" /gene_functions="biosynthetic (rule-based-clusters) porA: PFOR_II" /gene_functions="biosynthetic (rule-based-clusters) porA: Fer4_7" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_16" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255259.1" /locus_tag="CLOSPO_RS04685" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02262" /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase" /protein_id="WP_003484327.1" /sec_met_domain="PFOR_II (E-value: 2.5e-17, bitscore: 60.3, seeds: 27, tool: rule-based-clusters)" /sec_met_domain="POR_N (E-value: 4.1e-81, bitscore: 269.3, seeds: 31, tool: rule-based-clusters)" /sec_met_domain="POR (E-value: 4.6e-33, bitscore: 112.0, seeds: 86, tool: rule-based-clusters)" /sec_met_domain="TPP_enzyme_C (E-value: 4e-09, bitscore: 33.6, seeds: 323, tool: rule-based-clusters)" /sec_met_domain="Fer4_10 (E-value: 2.3e-07, bitscore: 28.1, seeds: 59, tool: rule-based-clusters)" /sec_met_domain="Fer4_7 (E-value: 2.4e-10, bitscore: 38.1, seeds: 208, tool: rule-based-clusters)" /sec_met_domain="Fer4_16 (E-value: 1.9e-09, bitscore: 35.5, seeds: 227, tool: rule-based-clusters)" /sec_met_domain="Fer4 (E-value: 6.6e-06, bitscore: 23.0, seeds: 528, tool: rule-based-clusters)" /transl_table=11 /translation="MRRMKTMDGNTAAAYISYAFTDVAAIYPITPSSPMAEHVDEWVAQ GKKNIFGQPVKVMEMQSEAGAAGAVHGSLQAGALTTTYTASQGLLLMIPNMYKIAGELL PGVFHVSARALAANSLNIFGDHQDVMAARQTGLALLAESSVQQVMDLSAVAHLSAIEGR VPFINFFDGFRTSHEIQKVEVLEYDELENLVDMDGVKAFRRRALNPDHPVIRGTAQNPD IYFQEREVSNNYYERLPEIVEKYMGEISKLTGREYHLFNYYGAEDAERLIIAMGSACDT IEEVVDYLMAKGEKVGLLTVHLYRPFSLEHFFKYIPKTVKNIAVLDRTKEPGALAEPLY LDVKNAFYGKEWQPTIVGGRYGLGSKEVYPSHILSVYENLKKDKPKDGFTIGIVDDVTN TSLEEAEAINTTPAGTTACKFWGLGSDGTVGANKSAIKIIGDHTDMYAQGYFAYDSKKS GGITISHLRFGKSPIQSPYLINQADFVACHNQSYVYKYNVLEGLKKGGRFLLNTIWTPE EVETHLPASMKKYIAENDIEFYTLNAVKIAQEIGLGGRINMICQAAFFKIANIISVEDA VKYLKDAVVTNYGKKGQKIIDMNNAAIDKGVNAIVKIEVPASWKDAKCEGACEVKENPE FIKNIVEPMNRQEGDKLPVSAFKGMEDGTFPSGTAAYEKRGIAINVPEWQLDKCIQCNQ CSYVCPHAVIRPVLLSDEEVKNAPEGFKSKPAVGAKGLNFTMAISPYDCTGCGNCADVC PAKEKALIMKPFDTQIEQDKNWEYAMKVSPKSNPMKKNSVKGSQFEQPLLEFSGACAGC GETPYAKLVTQLFGDRMMIANATGCSSIWGASAPSTPYTTNHKGYGPAWANSLFEDNAE FGMGMYLGVKQIRDKVTEDVKAVLGFKSAEELQSCAIGTEDCSEKDMTGTVISGELRAA LEDWLNNKDLGEATRERADKVIELVGKEKGSDKFLNEIYENKDFLVKRSHWIFGGDGWA YDIGYGGVDHVLASGEDVNILVFDTEVYSNTGGQSSKATPTAAIAKFAASGKKTKKKDL GAMAMTYGYVYVAQIAMGADKNQTLKAIAEAEAYPGPSLIIAYAPCINHGLKAGMGCSQ LEEKKAVDCGYWGLYRFNPELKEAGKNPFSLDSKEPTANFKDFLMGEVRYASLAKQFPE DAEALFAKTEQDAKERLENYKKLAEQ" gene 13281..13397 /locus_tag="CLOSPO_RS04690" CDS 13281..13397 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001388471.1" /locus_tag="CLOSPO_RS04690" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /product="hypothetical protein" /protein_id="WP_003361282.1" /transl_table=11 /translation="MKVMGRKMKKRRVEKTVNNMLDNVEKGFKKIAKEMNKK" gene 13654..14055 /locus_tag="CLOSPO_RS04695" /old_locus_tag="CLOSPO_02263" CDS 13654..14055 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255257.1" /locus_tag="CLOSPO_RS04695" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02263" /product="DUF1292 domain-containing protein" /protein_id="WP_003484330.1" /transl_table=11 /translation="MKDEKINSCGCGCGCEEEIEKDTCGCGEVEENCGCGCGDNHEEHH HEHCGCGCEDEECGETILVDLQDENGNAVTCEVIEEFEYKDNVYALVQNPENNSVYLFR EEGQGDEVELVNPDEKEFEEVTAYYENLQ" gene 14215..14856 /locus_tag="CLOSPO_RS04700" /old_locus_tag="CLOSPO_02264" CDS 14215..14856 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255256.1" /locus_tag="CLOSPO_RS04700" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02264" /product="HAD-IB family hydrolase" /protein_id="WP_003484332.1" /transl_table=11 /translation="MEKLAIFDVDYTLTKQETLIQFYKFMLKKKPSLIFHAPKIVISGL LYALKIFQAGKAKEIFIGFIDGITEDEMQSYVKEFYDKKLSKILYKDAIDTMRKLKSEG YKVYLISASAEFYLKELYNIKEVDKVIGTIFTLKEGSYKRKIIGENCKGEEKVRRLMEV LKEENIEVDFKESYMFSDSLADLPLFKLVGKPYLINAKKKYNNIEVLNWK" gene 14981..15814 /locus_tag="CLOSPO_RS04705" /old_locus_tag="CLOSPO_02265" CDS 14981..15814 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001388468.1" /locus_tag="CLOSPO_RS04705" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02265" /product="hypothetical protein" /protein_id="WP_003484334.1" /transl_table=11 /translation="MECFNEFFIENEKIKERGSFEESFLKEGKSLYEVIRIIDGAPLFL KSHLERFYNSAKLEGLNLWLDEEEIKENINRLIEINKVSIGNIKLVFNFNKGKNNNFLC YFLKHNYPEDIEYKRGVKTILYHGERENPNAKVINMDFRKIVGEKIKEEKAYEAILVDR NGYITEGSKSNIFMIKDGKVITAPIEKVLPGITRQNIIDVCKNLNLKVGEEKVHYKEIY KLEGLFISGTSPKVLPIKSVDEIEFKSSENKLILSIMEGYNKAIEEDVKGYKNKE" aSDomain 15107..15760 /aSDomain="Aminotran_4" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_CLOSPO_RS04705_Aminotran_4.1" /evalue="1.50E-43" /label="CLOSPO_RS04705_Aminotran_4.1" /locus_tag="CLOSPO_RS04705" /score="140.8" /tool="antismash" /translation="LFLKSHLERFYNSAKLEGLNLWLDEEEIKENINRLIEINKVSIGN IKLVFNFNKGKNNNFLCYFLKHNYPEDIEYKRGVKTILYHGERENPNAKVINMDFRKIV GEKIKEEKAYEAILVDRNGYITEGSKSNIFMIKDGKVITAPIEKVLPGITRQNIIDVCK NLNLKVGEEKVHYKEIYKLEGLFISGTSPKVLPIKSVDEIEFKSSENKLILSIME" gene 16069..16896 /locus_tag="CLOSPO_RS04710" /old_locus_tag="CLOSPO_02266" CDS 16069..16896 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255254.1" /locus_tag="CLOSPO_RS04710" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02266" /product="PRD domain-containing protein" /protein_id="WP_003484336.1" /transl_table=11 /translation="MENYTVKKVLNNNVIIANWQEEDAILVGKGIGFNSKKGETLSKDK IEKVYIKTTSKMSENYNKVLDNIDNRIVGISEEIINFSEKALNTKLNEGVHISLPDHIN FALRRIEKGINIENPFLEELGIIYPKEYEIAMKALNMINDRLKVNLPKDEAGFICLHIR AGITKQQVSESLAYTKKISKVMELISKLKGKPLDKNSLSYVRTLTHLNFMIERVKENKT IKNELLDSIKEEMKSEFGIAIKVAMLIEKLFEIKIPEDEIGYIALHLKRIGQY" gene 16979..18934 /locus_tag="CLOSPO_RS04715" /old_locus_tag="CLOSPO_02267" CDS 16979..18934 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255253.1" /locus_tag="CLOSPO_RS04715" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02267" /product="PTS glucose transporter subunit IIA" /protein_id="WP_003484337.1" /transl_table=11 /translation="MKNILAVLQRVGKSLMLPVSVLPAAALLLRLGYPDLLNNIYMLKA GDAIFSNLPLIFAIGVAIGLSEGEGIAALAAIVGQLILQGILDVGSSKAAKEAAIKIAA QRNMTLENFMNSKFYDDILRSTNIDLGVFGGIIIGIIAAIIYNKYKNIKLPSTIGFFGG KRFVLILTAIISFMFGMINVAIWPEVQKNIDTFARFASTSPLGPGFYAAGKRLLIPLGL HHIYYPPFLYQFGSYVDPSGIKYFGDFSRYFHGDPTAGVFMASEFPILMFGLPGAAIAM ICAAEKNNRKKVLSIMLSAALVSFFTGITEPIEFAFIFVAPILFIFHVLAAFTSGIVTS IFNIRLGYTFSASFIDYILGYKFSGNGLLIFPIGIFYFLLYFIVFYYLIVKKDIKTLGR EGTVLKENKEIKSNEKAALILEALGGEKNITNLDCCITRLRVALKDEKKLDRVYLEKIN LLGIVQTGKVVQIILGTEAENIKYSIEQIIKKGMNPKEIIEYERSLKLMNPMEGEIVLL EQVPDEVFSEKLLGDGFAIKPYRNKVYSPIDGTIKFLFPSNHALAIETKEGLEILIHIG IDTVKLNGEGFKVYIKEKEKVKKGQLLVSYDKEFLEGKVKSLISPIVITNLKEDSEIKI EYGYKKEKEIVAYIKN" gene 19178..21211 /locus_tag="CLOSPO_RS04720" /old_locus_tag="CLOSPO_02268" CDS 19178..21211 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255252.1" /locus_tag="CLOSPO_RS04720" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02268" /product="DUF342 domain-containing protein" /protein_id="WP_003484339.1" /transl_table=11 /translation="MKKTIYSCSTLDKCINKACKELGVKEEELNYEIIEEKQGFFMKKT TILVNAEEKLMNMKEDKVSIEDSVEENIEHNDEKIEIEKESIAKESIAANDGKVKIVKN QVIVTNPKEGGKPAAIAPGDNITILVDGDRIKSRKEVFEENKIEVIFEEVKAHRELNVK TDQNNMEAYISVIYKPEIKYGSKDIEDKNYLILNSCKIEEKDPPYYTEEEIIEALKKMG IVYGIVKENLEQCTKNNCIELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVD FKSIGSIESVKPGEVLAVRKKGKEGQDGIDIKGIVKKHKQGKKIILKPGQGTVLRDEDT IEAAMEGKPCVKSNIFYVYQVHEVKSDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSV EIKKHVERSEIISKSNLNIDGNIINSDIYGGGEDTLKVMVLNKLEKLKDTLTELISAVE EIKRFNLLGEGKKDGEIIKILIENKFRSLTKICIAIMANINMCRSDDEEDELVGIIRKK LIGLGPVHIKNYRELEQIVNLIEEKMKMCKDGLSLPVNVNISYCQDSKIQSTGDIYITG KGEYISKLTSNNNVYFTKDGSVARGGHITAKNEIRCKEVGSEAGVITKLQILQKGDIYV DVAYQNTVFIIGDREYLLETPSKDIHAYLDKKGEITVEKFVL" gene 21227..21673 /locus_tag="CLOSPO_RS04725" /old_locus_tag="CLOSPO_02269" CDS 21227..21673 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255251.1" /locus_tag="CLOSPO_RS04725" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02269" /product="chemotaxis protein CheW" /protein_id="WP_003484341.1" /transl_table=11 /translation="MDISEAKVLIFKINGEYYAADIMEIERILGYEEPTNLPDAPSFVQ GVINYQGKILPVVSLSRKFNLKEEEIKNEAKIIVVKEEENKLGIIVDVVSEVKDVKLDN IEQPPEIVAGISKRYIKGLIKIENKIIIFLNLSTILTEEEKSMI" gene 21690..22178 /locus_tag="CLOSPO_RS04730" /old_locus_tag="CLOSPO_02270" CDS 21690..22178 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255250.1" /locus_tag="CLOSPO_RS04730" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_02270" /product="chemoreceptor glutamine deamidase CheD" /protein_id="WP_003484344.1" /transl_table=11 /translation="MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCVGGL SHIMLPDSTQFSKVTNPMKFADLAIPILVEKMEKLGARKNGLKAKICGGASMFNFSDKS MVMDIGNRNGKAVKEKLKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTKEI" ORIGIN 1 atgagtaatt tgtatgaaaa gtataaaaat gctagagttc atgcccctaa tttcccaatg 61 actattgaat ggataggtgc caaaaagaca aatttggata aactaaaaaa tagagttata 121 gttttagatt tttggacttt ttgttgtata aattgcatac atgtaataga ggatctaaaa 181 tatttagaag aaaaatataa gaataagtta caggttatag gagtacattc tcctaaattt 241 aaatatgaaa aaaacagtaa agctatatta aatgctatga aaaaatatgg aataagtcat 301 ccggtagtga atgataaaaa taaaagtata tgggatagtt atacagtaaa tgcatggcca 361 acatttgtat taatagatcc agaaggatat gcctttgcta tggtatcagg agaaggtaat 421 agagaattat tggatcaaat aataggtgac acaatagaat actttgataa tatacattgg 481 tcagaagaaa acatagtttt gcaaaagaaa tgtgaaaaag aagaatatat atatccatca 541 aaaatatcta taaatgaaaa tggattaata gccttttcag aaaaaggtag gaatagaata 601 gtaatattag atcaaaactt agaaaaaata aaaacaatag gtagtagtga atatggatta 661 aaggatggag attttaaaga agctcaattt aaagctccag aagggctaag atttatagaa 721 aataagatct atgttgcaga cactgaaaac cattccataa gagaatgtga tttagaaaag 781 gaaatggtta aaactatagc aggtaatgga caaaaggaat atagtcctat ggcaaaggga 841 gatgctttga atgtaagttt aaattctcct tgggatttag aaatattaga agactgtata 901 tatatagcta tggcaggaaa tcatcaaata tggaaatata atatgaaaga taaaagtata 961 gaaagtcata taggtacagg aggagaaaac ataagagatg gaagctttga taaatgttta 1021 ttagcgcaga catcagctct atgttacgat ggaaaaaaaa gactatactt tgtagattca 1081 gaaaccagtt ctataagata tgcagattta gaagaacata aagttcatac acttatagga 1141 aaaggattgt tttattttgg aagcaaagta ggaagttttg aaaataccat gcttcagcat 1201 cctttagact taaaatataa agacaatgta ttatatatag cggacacata taacaataga 1261 ataataaaag cagatatttt aaatgaaaaa gtagaaataa taaaaaaagg attaaatgag 1321 ccaaatggaa tagctatata tgaagatagt atatatatgt gtaatacaaa taatggcaaa 1381 attgaaaaaa taaacatgaa ataaggaaag tgcatagcac tttccttaaa gttcgtaata 1441 ggaattttta agaagaaggg catccttttc aagtattgga ctttcacata ttatacaacc 1501 attgatatta aaatctttaa aagctttaag gcaagcaata tagttaaagt cgctttcctc 1561 aaaaggaaga tggtttcttt cacctttttc actataatgt attccagaaa gatggatatg 1621 catatcatct aaagcagatc ttcctagatt tttttctaca taacttaata tattataaaa 1681 atccttatag gtttttaaac caccattaga cctagcatgt atatgagaaa agtctataca 1741 taatttgcaa gtggcaactt ctttacatag ggctacattt tcttctaaag aaccaaattg 1801 agttcctttc ccagtggttt ccaatctata atctactcct aaatctggta atttttttaa 1861 gttttctaaa atagttttaa aagtttcttc tttggagtcc ttaagataaa atccaggatg 1921 aaatattaaa cttcttccct gaactttttt taagccctca gcacctttta ttattctttc 1981 caatgatttt tcttgctttt ctatttcatc tgcgtttaga tttataaaat aggaaccatg 2041 ggcagataaa taaaaattac tattattttt ttcctctaat atgccttctt tgtttttatc 2101 tgtaacatta acagatctta caaagggtaa ttccatggca tctaagccta tattgtttaa 2161 atatgttata ccagatttat aattaaattt agtagttcca tcacctatgg gtaggccgga 2221 tattccaaaa agtaatctgt ccataaataa aacctccatt gcgttaattt aatatacata 2281 ttattatgaa aaatataatt taaaactaat ttgataatga ttattatata atatttataa 2341 gagtatgtaa aatcagaatt aaaattgaga taaataaaat gaatacaaaa gtattaacct 2401 taatggtata attagcaagg aagaggtgtg tggcttgaag aaaaagtgga tgttaagaag 2461 aagtaaattg aatataaaag atatagccca taagtcaggt ataagtgaaa ctttatgtac 2521 tatattagtt aatagagaaa tatataattt agaagatata gagagttttt tgaatccttc 2581 tttaaaaaaa ctatataatc ctttattaat gaaagatatg gacaaaggta cagaaataat 2641 aaaaaaggca ataatagata aaaagaaaat agccatttat ggagattatg atgcagatgg 2701 tgttactagt accgtaatat tatacactgc tttaaaggaa tgcgaagcta atgtgactta 2761 ttacatacca gatagagaaa cagaaggata cggtatgtgt acagacagag ttgaaaaatt 2821 aaaagaagag ggtgtagagg ttataattac ctgtgataat ggtatagctg cattagagca 2881 agtggaacga gcaaaagagt taggtatgac agtggtaatt acagaccatc atgaattacc 2941 ttttatagaa aacgaaaatg gtgaaagaga atatgtagta ccaaaagcag atgttatcat 3001 aaatccaaaa caaaaggatt gttattatcc ctttaaaatg ctttgtggag caggcatagc 3061 ttttaaattt tcaaaacttt tatatgaaaa actaaatatg aatcctaata aatataaaga 3121 attattagaa tttgcagcta taggaaccat atgtgatgta gtagatttaa aggatgaaaa 3181 tagaataata gctaaattgg gattaaaaaa cataaataat acaaataacc tagggctaag 3241 agcacttata aaagagactt cattaaatgg aaaaaatata acttcttata atgtaggatt 3301 tataataggg ccttgtataa atgctacagg aagattagat acagcggctc tttccgtgga 3361 gttatttttg accaaagata taaaaagagc agaggaacta gcaaaagagt taagaaactt 3421 aaataccgag agacaagaaa taactatgga aggtgtagag gaaataacat ataccataga 3481 aaattcatca ttaaagaatg ataaagtatt agttatttat aaagaagata tacacgaaag 3541 tatagcaggt atagtatcag gaagaataaa ggatgcatat aatcgcccaa caatagtttt 3601 aactaaagga aaagaaatgc ctaaaggatc tggaagatct atagatggat acaatctttt 3661 tgaagaactt atgaaatgca aggaatacat atataaattt ggtggccatc caatggctgc 3721 aggccttact atagaagaaa aaaacataga taaaataaga gaagaattaa acaaaaattg 3781 caatttaaaa gatgaagatt ttatacctaa aataagaata gataaaagac tacctttaaa 3841 agaagtaaac ttagagttaa tagatagctt acagtgttta gaaccttttg gtaaaggaaa 3901 tagttcacca atactagcag aaaaaaatat aaagatagaa ggaataagaa tactaggcaa 3961 agatcaaaac acattaaaat taacttgtag aatagaagat acaaatagaa gaatagatgc 4021 aatagccttt ggaaaagtgg atgaatttaa aagagattta gaaaatatat atggagatga 4081 aagtatagaa aatattataa ctaacccact tatggcaggt ttaaaattag atttaatata 4141 ctatccaatg attaatgaat acaacggaaa tgtgtacacc caattaagaa taatagacta 4201 cagaataaat agataaaata aggaaagtgc gacgtatttt ccttaaagaa cagagtatag 4261 ataatagaga acagatatgg atgatttttc tccgctttgc tacgtaaaat gtaaaattat 4321 attttctttt tttataaaaa agatttaaga tttcaaaaaa attttcgata gaaaattaat 4381 gcgtagcatg gccaaagatt gtacttttca taatctttat acgaatctaa attgcattca 4441 aaactatttg cgttagcaaa caaatttaat atataaagcc actaatttca gctagttttt 4501 atataaactt aatgtaatca gtggctttta tatatttttt tagttatact cctctaatga 4561 agaaaatttt taatattact ctatataaaa ttcttataaa tgtttagcta caaaacttct 4621 gactaagccg ataattgtaa atcaagcttc tgcttaattc tttgcataag taagcaccca 4681 tgtaaaaatt ataggtttgg gatatttact taaatttttt atattaatct aaatattaat 4741 tgctatagaa agcctgcgga cgcgctatca cttttcttaa aatatctata tatgaaatga 4801 aaatttaatt ttataatatt taaactactt aataatttta gtaaaaatat ttaaattaaa 4861 gattttgtgt agaggcgcgg aacaaaatca tctacagctg ttctctgttc attatactct 4921 gtactctaca taagaaaagt gagtggcact ttccttagaa gttgactaag ggcgtagaaa 4981 aagagtaaac tatataaagt gatatataat aatagggggg aaatatggga ccaataggaa 5041 gtgttttttt ggaagataaa aatacaataa aaagtataat ggatagcatt ccttattcat 5101 gttatattgt aaacgataaa tatgaattgc tttatgtaaa tagtatagct ctaaagttaa 5161 gcaaagaact aaaaagaaaa tctagttcta agctacggcc tggagaagaa aaaacattga 5221 tggattgttt agatgaatac gaaataaatt ttatcgatgg tgaacctgtt acagaaaaaa 5281 actttcctgt ttttatagct cttaaagaac aaaagaagat aaaagaagtt tcattaatat 5341 ttacaaaaaa caattgtgaa aaggtatgtt ttagtttaac tgtaatacct ataaatttag 5401 aaggaaccaa aaagggagct ttagtaaatt taatggatat tactcaagag cacacctata 5461 aagaaaccat agaaaaagat aaggaagaaa ttttaaatat ttcagaacaa ataaaaaata 5521 agagtgaaat tatagacata ttaagaaaaa aagaattgga ttacataaaa tatcttaata 5581 atgtaattaa taatgtatct gaaggaatat tggtttttga taagcattca aaattaattt 5641 tatggaataa agctattgaa caaataggtg gaatgaaaat taaaaaaata ttgggcatta 5701 aagaaataat taatgagtat gatatataca tactagataa tcataaaaat atgaaatata 5761 aaattaaaaa agtaaatcca gattataata gtagtgtaaa agacctatta ttgaaattgg 5821 taaaaaaaga tagtaatgag attaagtata tagaatataa tagtaatcct attatagatg 5881 aaaataatga gatactatat actatatcaa gtataaagaa tgttacagat ttaatagaaa 5941 gtcaaataat atctgaagaa agagcagaat ttataaaata tgtagtagat tgtatagagc 6001 taccattagc agtattagat tatcctgatt taacatataa attagttaac gaaagatatg 6061 aggaattagt agaacgatta tatactaaaa aaataaaggg taatttactt ggaaatacag 6121 tggaagatgt ttttagaaaa gaagaattta aagagcagtt agaagtatta cagaaaatga 6181 aagagggaac tattaaagag tatacatttt cacctgaaaa atttgttcta cagtcaggag 6241 aagaaagatt ctttaaagta aaatatattt atcatgaaac taaaaataaa aaaagagttc 6301 atatacatgg ttctgatgta acagaagaat ttaaaaataa tttggaacta gaaaaggtaa 6361 ataagcttaa agatgagttt tttactataa tatctcatga attaagaact ccgttgacca 6421 taatatattc ttccctacaa ttagcttatg atatttattc aaaggatata aacccaagta 6481 tcgataagat tttaaaaaga ataaatcaaa atacagctag acttttaaaa cttataaata 6541 atatactaga tatatcaaaa gcagaagcgg gatttttagc tttaaataat gaatattttg 6601 atgtagtatg ggaaacagaa aatatagtta attcaataac aaattatgct aattctaaaa 6661 atataaattt aatatttgat actacaaaag aagaagaaaa ggtttggatt gataaagata 6721 aatatgaaag aatattatta aatttattat ctaatgctgt aaaatttaca ccagaaaaca 6781 aatctatata tgttactctt aaaatatata aaaataattt taaattaata gtaaaggatg 6841 aaggggcagg aatacctaaa gaaaaaatag attgcatttt taatagattt gctcaagtga 6901 atagttcatt atcaagaaat gcagaaggaa caggtatagg actttcatta gttaagaaaa 6961 ttgtggatct tatgggtgga gaaataaaag taaatagtac tctaggaaag ggaactacat 7021 ttactgttaa aataaataaa aatatggata aatgttatga aaagatagag aaaactgtta 7081 tacacaatga tatttctaaa aaaatacata tagagttttc agatataaac tagaaaatat 7141 ataaaaccct atttaggtaa ataaaattta caattaaata gggcttttat tattaatcta 7201 taatagagtt ttaaagaaat attgtgaatt ttatatcatt attcgtttaa aatttatatg 7261 gattaaaaga ttcatctgaa ttaagtttta ctttattaga aaaattttta ggattattaa 7321 aatttgtaga acctatttcc atatcttttg agctttctat tctaaatcta tttaaagctt 7381 cttttacttc tggcactaaa ttttttgtca ttattagcac ctcctaatat aatatagaat 7441 atttaaatat atagaattaa taatatatta accaataata atttatatat attataatat 7501 taaaggtaaa aaatataaat tatacataga atgaaaaagt atttaaaaaa ataatattta 7561 tagtattatt aataatgagc tttatatgtg tataataaat atgatgaaaa accttagaag 7621 ttttggatta agtgcatatt tttttctttt gaataaactt tattaaaagt aacaataatt 7681 ttacaaaaaa ataaaaacta gattatatac ataatgaatt aaagaaaggg gatataattt 7741 tgaaaaagat tataatgaca ggtggaggaa cagcaggcca tgttacacca aatttagctt 7801 tagttccaga attgaagaaa ttaggatatg aaattaagta tatagggagc atagaaggaa 7861 tagaaagaaa aattataaaa aaagagggta tagaatactt ccccatttct agtggaaaat 7921 taagaagata ttttgactta aaaaatttct ctgatccatt taaagtgtta aagggagtgt 7981 ttcaagcaaa aaaaataatt aaaagagaaa aaccagatat aattttttct aagggaggat 8041 ttgtaactgt accagtggtt atagcagctc atttaaataa aataccagtt atagctcatg 8101 agtcagatat aactcctggt cttgcaaata aattagctac tccttattgt acaaaagtat 8161 gcgttacttt cccggagtca ataaaacata taaaaggaga caaagctgta ttaacaggta 8221 cacctataag aaaagaattg ttagaaggaa gcaaagttaa aggcatagaa ttatgtggat 8281 ttaaagatga taaacctatt cttttaataa ttggaggaag tttaggttca aaaattataa 8341 acgaaatagt cagaaaaaat ttggatgata tacttttaaa attcaatata atacacattt 8401 gtggaaagtc taacttagat gaaaatttag aaaatagaaa gggatatgcg caatttgagt 8461 atgtaaatga agaattacct gatttaatga aagcgtcaga cttggtaata tcaagagctg 8521 gagcaaatgt aatatatgaa ctgttagcgc ttaaaaagcc aaatctttta atacccttat 8581 ctaaaaaatc tagtagagga gaccaaattt taaatgcagg ttccttcgaa aaaagtggat 8641 atagtttagt tttaaaggag gaggagctat cagataaaac tttaatggaa aagttaagtt 8701 acttgtatga aaacagaaat gtatatataa ataatatgtc caaaagtaaa atggataatg 8761 gcgttaaaaa tataacagaa ttgataaaaa aatatacaaa ataaacatat gatattaaaa 8821 ttaaagttaa attaaattaa tcctgcatag tttaataaat tcaaaaaaat atatatatta 8881 agttaaatta tagaataata attctataaa attattaaat gttaatgtat atatattatt 8941 tttttatttt aatgttaaat gggaagatct ttaaaaatat accattgaat ttatttattt 9001 aagtattata ataaaaataa gcaacaaatt tttgaagctt ataaaaaacc ttataaataa 9061 gcctttaaaa atttgtttct tagtcatctg ctttatggtc ttaccagtac tggtaagaac 9121 gtagtaagac aaaatatata caattttata ataaaattta tatgttttta attctgtata 9181 tgctggaata aaaatgtatt agtgctataa tgtgctttgt aggaaagcta tgaatttttc 9241 gcaatttaaa atgattttta tgctttaaag tgttatttta tgcataaaca ctgaaaagga 9301 ttttacagat aaaaattttt ggtgtatatt aataatgggc tttgaagaaa aaggtcaaat 9361 tttacaaatg ttaattattt tgcggattta ttccgtaaat tttatataaa ttaaagggaa 9421 agaagggaat tttctatgag aagaatgaaa actatggatg gtaatactgc tgcagcatat 9481 atatcatatg catttacaga cgtagcagct atttatccaa taacaccttc atcaccaatg 9541 gcagaacacg ttgatgaatg ggtagctcaa ggaaagaaaa atatatttgg acaaccagta 9601 aaagttatgg aaatgcaatc tgaagctggt gcagcaggtg ctgttcacgg ttctctacaa 9661 gcaggtgcat taacaacaac ttatacagca tctcaaggtt tattacttat gatccctaat 9721 atgtacaaaa tcgctggaga acttttacca ggagtattcc acgtaagtgc aagagcatta 9781 gcagccaatt ctctaaacat atttggagac catcaagatg ttatggcagc tagacaaaca 9841 ggactagctc tattagctga aagcagtgta caacaagtta tggatttatc agcagttgca 9901 catttatcag ctatagaagg aagagtacct ttcataaact tctttgatgg atttagaaca 9961 tctcatgaaa ttcaaaaagt agaagtttta gaatatgatg aattagaaaa tttagttgat 10021 atggatgggg taaaagcttt cagaagaaga gctttaaatc cagatcaccc agttataaga 10081 ggtacagctc aaaaccctga tatttatttc caagaaagag aagtatctaa caactactat 10141 gaaagacttc ctgaaatagt tgaaaaatac atgggagaaa tctcaaaatt aacaggaaga 10201 gaatatcacc tattcaacta ctatggtgca gaagatgcag aaagattaat aatagctatg 10261 ggatcagcat gtgatactat agaagaagtt gttgattact taatggctaa aggagaaaaa 10321 gttggtttat taacagtaca tttatataga ccattctcat tagaacactt ctttaaatat 10381 ataccaaaaa cagttaaaaa cattgctgtt ttagatagaa ctaaagaacc aggcgcttta 10441 gcagaaccat tatacttaga cgttaaaaat gcgttctacg gaaaagaatg gcaaccaaca 10501 atagttggag gaagatacgg attaggttca aaagaagttt atccatcaca catattaagt 10561 gtatatgaaa acttaaagaa agataaacca aaagatggat ttactatagg tatagttgat 10621 gatgttacaa atacatcatt agaagaagca gaagctatca acacaactcc agctggaact 10681 acagcatgta agttctgggg attaggttca gatggtactg ttggagctaa caagagtgct 10741 ataaaaatca taggtgacca tacagatatg tatgcacaag gatactttgc atatgattct 10801 aaaaaatctg gtggtataac aatttctcac ttaagatttg gtaaatcacc tatacaatca 10861 ccatacctaa taaaccaagc tgactttgta gcttgtcaca atcaatctta tgtatataaa 10921 tacaatgtat tagaaggatt gaaaaaaggt ggtagattct tattaaatac tatctggact 10981 ccagaagaag tagaaacaca tttaccagct tcaatgaaaa aatacattgc tgaaaatgat 11041 atagaattct atacattaaa cgctgttaaa atagcacaag aaataggatt aggcggaaga 11101 atcaacatga tttgtcaggc tgctttcttt aagattgcaa acattatatc agtagaagat 11161 gcagttaaat acttaaaaga tgccgttgta actaactacg gtaaaaaagg tcaaaaaatc 11221 attgatatga ataacgctgc tatagacaaa ggtgttaatg ctatagttaa aatagaagtt 11281 ccagcttcat ggaaagatgc taaatgcgaa ggtgcatgtg aagttaaaga aaatccagaa 11341 tttatcaaaa atattgttga accaatgaac agacaagaag gagataaact tcctgtaagt 11401 gcattcaaag gaatggaaga tggtacattc ccatcaggaa cagctgctta cgaaaaaaga 11461 ggaattgcta taaatgttcc agaatggcaa ttagataaat gtattcaatg taaccaatgt 11521 tcatatgttt gtccacatgc ggttataaga ccagtacttt tatctgatga agaagttaaa 11581 aatgctccag aaggattcaa atcaaaacca gctgtaggag caaaaggatt aaacttcaca 11641 atggctataa gcccatatga ttgtacagga tgcggaaact gtgcagatgt atgtccagct 11701 aaagaaaaag cattaataat gaaaccattt gatactcaaa ttgaacaaga taagaattgg 11761 gaatatgcaa tgaaagtatc tccaaaatct aacccaatga agaaaaattc agttaaaggt 11821 agccaatttg aacaaccatt attagagttc tcaggtgcct gtgcaggttg tggagaaact 11881 ccatatgcta aacttgtaac tcaattattc ggagatagaa tgatgatagc taatgctaca 11941 ggttgttcat caatttgggg agcatcagca ccatcaactc catatactac aaaccataaa 12001 ggatacggtc cagcttgggc taactcatta ttcgaagata atgctgaatt tggaatgggt 12061 atgtatcttg gagtaaaaca aataagagat aaagttacag aagatgtaaa agctgtatta 12121 ggatttaaat cagcagaaga acttcaaagc tgtgcaatag gaacagaaga ttgttcagag 12181 aaagatatga caggaacagt tatatcagga gaattaagag ctgctctaga agattggtta 12241 aacaataaag atctaggaga agctactaga gaaagagcag acaaagttat agaattagta 12301 ggaaaagaaa aaggaagcga caaattccta aatgaaatct atgaaaacaa agatttctta 12361 gttaagagat cacactggat attcggtgga gatggttggg cttatgacat cggatatggt 12421 ggagtagacc acgtacttgc ttcaggagaa gacgtaaata tccttgtatt tgatacagaa 12481 gtttactcaa acacaggtgg tcaatcttca aaagcaacac caactgcagc tatagctaag 12541 tttgcagcat ctggtaaaaa gactaagaaa aaagacttag gtgcaatggc tatgacttat 12601 ggttatgttt acgtagcaca aatcgctatg ggagcagaca aaaaccaaac attaaaagca 12661 atagctgaag ctgaagcata tccaggtcca tcattaataa tagcttatgc tccatgcata 12721 aaccatggat taaaagcagg aatgggctgt agccaattag aagaaaagaa agctgttgat 12781 tgtggatatt ggggattata cagattcaac ccagaattaa aagaagcagg aaagaatcca 12841 ttctcattag attcaaaaga accaacagca aacttcaaag acttcttaat gggagaagta 12901 agatatgcat cacttgctaa acaattccca gaagatgctg aagcattatt tgcgaaaaca 12961 gaacaagatg ctaaagaaag attagaaaac tacaaaaaat tagctgaaca ataataaaaa 13021 gagccgcaag gctctttttt attaagtata aagagcgata ttactttaaa atttatagct 13081 atattacaaa aaactagcat gagtgtaagt tcaatacaaa caataagttt ttttaatttc 13141 ctatttaata tagtattaat gattttagct ataattattt gtgtaaaatt aatagaaaac 13201 aggcaaatat ttataaagtt caaatagcat ataaaaaaag aaaatacata aaataaagct 13261 ataacttctt ctgaagggag atgaaagtta tgggaagaaa aatgaaaaaa agaagagtag 13321 aaaaaacagt aaacaacatg ttagataacg tagaaaaggg ttttaaaaaa attgctaaag 13381 aaatgaacaa aaaataaata aaaaattaaa tactacaaaa agtaaataaa aatatttcat 13441 taccaagtaa atagttaata tcaattattt acttggtata tttttatcat aaaatatttt 13501 ttgaatttat aaatatacaa agaaaatgat aatgattatt aaataatttt atgaaaaagc 13561 aaaaaaaccc ttttaaaaaa ctctgtttaa gggtacaatt agaacatagg aaaaaaggag 13621 gtgaagatcc tggtaatatt atcaggagat tttatgaaag atgaaaaaat aaatagttgc 13681 ggttgcggtt gtggctgcga agaagaaata gaaaaagata cttgcggttg cggtgaagta 13741 gaagaaaact gcggttgtgg ttgtggtgat aaccatgaag agcatcatca tgaacattgc 13801 ggttgtggct gcgaagatga agaatgcggt gaaacaattc ttgtggattt acaagatgaa 13861 aacggaaatg ctgttacttg tgaagtaata gaagaatttg aatataaaga taatgtatat 13921 gcattagtac aaaacccaga aaataattca gtatacttat ttagagaaga aggtcaaggg 13981 gacgaagttg aacttgttaa cccagatgaa aaagaatttg aagaagtaac tgcttactac 14041 gaaaatttac aatagtatta taaaaagagt ctctgatttg tcaggggctc ttttagttta 14101 ataggaaata ttaggattaa aaagtttagt ataaataaaa aagtgaatat attataaaaa 14161 atcacaaaat atatagtata atataagaat acaacttata gaagaggtgg tattttggaa 14221 aaattggcaa tatttgatgt agattataca cttaccaaac aagaaacact tatacaattt 14281 tataaattta tgcttaaaaa gaaaccctct cttatatttc atgctcctaa aatagttata 14341 tctgggcttt tgtatgctct aaaaatattt caagcaggaa aagctaaaga aatatttata 14401 ggatttatag atggtatcac agaggatgaa atgcaaagtt atgtaaaaga attctatgat 14461 aaaaaattaa gcaaaatatt atacaaagat gctatagata ctatgagaaa attgaaaagt 14521 gaaggctata aagtatacct tatatctgct tctgcagaat tttatttaaa agaactttat 14581 aatataaaag aagtagataa agtaatagga actatattta cattaaaaga agggtcatat 14641 aagagaaaaa taataggaga aaactgtaaa ggtgaagaaa aggtaagaag gcttatggaa 14701 gttttaaaag aagagaatat agaagtagat tttaaggaat cctatatgtt ttcagattcc 14761 ttagcagatt tacctttatt taaattagta ggaaagccgt atcttataaa tgcgaaaaag 14821 aagtacaata atatagaagt acttaattgg aagtagagta aaatttaatt catatttaat 14881 ttactaactg taagtttaaa ttgaaactaa tttaagcatt tataatatta atacacaata 14941 taagaattgt gatgtttaca gtaaatgggg ggatatgtta atggaatgtt ttaacgaatt 15001 ttttatagaa aatgagaaaa taaaagaaag aggttcattt gaggaaagtt tccttaaaga 15061 gggaaaatct ctatatgaag ttataaggat aatagatgga gcgcctttat ttttaaaaag 15121 ccatttagaa agattttata actctgcaaa attagaagga ctaaatttat ggttagatga 15181 agaagaaata aaagaaaata taaatagact tatagaaata aataaagtat ctataggcaa 15241 tataaaacta gtatttaatt ttaataaggg aaagaataat aactttttat gctatttttt 15301 aaaacataat tatccagagg acatagaata taaaagggga gtaaaaacta tactttatca 15361 tggagagaga gagaatccaa atgcaaaggt tataaatatg gattttagaa aaattgtagg 15421 agaaaagata aaggaagaga aagcttatga agcaatacta gtagacagaa atggatacat 15481 aacagagggt agtaaatcta acatatttat gataaaagat ggtaaagtaa taacagcacc 15541 tatagaaaag gtattaccag gaataacaag acaaaatata atagatgtat gtaaaaatct 15601 aaatttgaag gttggagaag aaaaagtaca ttataaagaa atatacaaat tagagggatt 15661 atttatatca ggtacatcac caaaggtttt accaataaaa tctgtagatg aaatagaatt 15721 taaatcttca gagaataaat tgatactaag tattatggaa ggctataata aagccataga 15781 ggaagatgta aaaggttata aaaataaaga ataactactt taaaaatcaa atcaatattg 15841 tggtgtgatt ttataaggta gatagcgtag caggatttgt taatacattg acaaaatatt 15901 ggataaaaaa tataatttta tattatgata taaaaattag ttcatttcta aagactttgt 15961 aatagaaaaa ttaataaatt ttataattat aacatagagg taggagtaga agatttaata 16021 ttatgttaaa tataagtttg gaaagaaaaa ggtttggagt gttaagattt ggagaactat 16081 acagtaaaaa aggttcttaa taataatgta ataatagcta attggcaaga agaggatgca 16141 atattagtcg ggaaaggtat aggatttaat tctaaaaaag gagaaactct ttctaaggat 16201 aaaatagaaa aagtatatat aaagaccacc tcaaaaatgt cagaaaacta taataaagtt 16261 ttagataata tagataatag aatagtaggt atttccgaag aaataataaa tttttcagaa 16321 aaggctttaa atacaaaatt aaatgaagga gttcatattt ctttgccaga ccatataaat 16381 tttgcattaa gaagaataga aaaggggata aatatagaaa accctttttt agaagaacta 16441 ggcataattt atccaaagga atatgaaata gctatgaagg ctcttaatat gataaatgat 16501 agattaaagg ttaaccttcc aaaggatgaa gcgggattta tatgtcttca tataagagca 16561 ggtattacaa agcagcaggt ttcagaatcc ctagcctata ctaaaaagat aagcaaagta 16621 atggaactta tatctaaatt aaaaggaaag cctttagata aaaattctct ttcttatgta 16681 agaactttga ctcatttaaa ttttatgata gaaagagtta aagaaaataa aactataaaa 16741 aatgagctat tagatagtat aaaagaagaa atgaaaagtg agtttggtat tgccattaaa 16801 gtagccatgc ttatagaaaa attatttgaa ataaaaatac cagaggacga aataggatat 16861 atcgcattac atttaaaaag gatagggcaa tattaaggta tattaaatat ataaactaaa 16921 gaaccttgct tttgcaaggt ttttaaagta taagctagct taaaggtggt gtgtaaaatt 16981 gaaaaatatt ttagctgttc ttcagagggt aggaaaatct ttaatgttac ctgtatccgt 17041 attacctgca gcagctctac ttttaagatt agggtatcca gatcttttaa ataatatata 17101 tatgttaaaa gcgggagatg ctatatttag taatcttcca ttaatatttg ctataggagt 17161 ggctataggc ctttcagagg gggaaggaat agcagcttta gctgctatag tagggcaatt 17221 aatattgcaa ggcatattgg atgttggaag ttcaaaggct gctaaagaag cggctataaa 17281 aatagctgcc caaagaaata tgacattaga aaattttatg aatagtaaat tttatgatga 17341 tatattaaga agtaccaata tagatttagg tgtttttggc ggaataataa taggaattat 17401 agctgcaatt atatacaata agtataaaaa cataaagcta ccaagtacca taggcttttt 17461 tggaggaaaa cgttttgtgc tcattcttac agctataatt tccttcatgt ttggaatgat 17521 taatgtggca atatggccag aagtacaaaa aaatattgat acttttgcta gatttgcaag 17581 cacatctcct ttagggccag gtttttatgc agcaggaaaa agacttttaa ttccactggg 17641 acttcaccat atatactatc cacctttttt atatcaattt ggtagttatg tagatcctag 17701 tggaattaaa tattttgggg atttctcaag gtattttcat ggagatccta cagcaggagt 17761 ttttatggct tcagagtttc ctatattaat gtttggcctt ccaggagcag ctatagctat 17821 gatttgtgct gcagaaaaaa acaatagaaa gaaagttctt agcataatgt tgtctgcagc 17881 tttagtgtct ttttttacag ggattacaga gcctatagaa tttgctttta tatttgtagc 17941 tcctatactt tttatttttc atgttttagc agcatttaca tcaggtatag taactagtat 18001 ctttaatata agacttggtt atactttttc agcctccttt atagattata ttttaggata 18061 taaattttca ggtaatgggt tattaatatt tcccataggt attttttatt ttttattata 18121 ttttatagtt ttttactatt taatagtaaa aaaggatata aaaactctag gtagagaagg 18181 tacagtactg aaggagaata aagaaataaa aagtaatgaa aaagcagctt taatattaga 18241 agctctagga ggagaaaaaa atataacaaa tttagattgt tgtataacca ggcttagggt 18301 agctttaaag gacgaaaaaa aattagatag ggtgtattta gaaaaaataa atttattagg 18361 tatagtacag acaggaaagg tagtacagat aatattaggt acagaagcag aaaatataaa 18421 atatagtata gaacaaataa ttaaaaaagg aatgaatcca aaggaaataa tagaatatga 18481 aagatcatta aaattaatga atcctatgga aggagaaata gttttattag aacaggtacc 18541 agatgaggtt ttttcagaaa aactattagg agatggattt gctataaagc cctatagaaa 18601 taaggtatat tcccctattg acggaactat aaaattttta tttcctagta atcatgcctt 18661 agctatagaa actaaagaag gtttagagat tttaatacat ataggtatag atactgtaaa 18721 acttaatggg gaaggattta aagtttatat aaaagaaaaa gaaaaggtta aaaaggggca 18781 acttttagta agctatgata aagaattttt agaggggaaa gttaaaagct taatatcacc 18841 tatagtaata acgaatttaa aagaagattc agaaataaaa atagaatatg gttataaaaa 18901 agaaaaagaa atagtagcct atattaagaa ttaatatgaa aattgttaaa attataataa 18961 ttattttata attagtctct acattattta ttaagaatta cgaaaataat aaaagagtat 19021 gtttaattta ttaagtatga tatttccttt gtaaataatt gaaataaatt gtaataaatt 19081 attatatgat ataaaatata gttatatata tctatattta gaatttatat attcctcaaa 19141 atagggggcg ggataatata atttaagggg gattaatatg aaaaagacaa tttatagttg 19201 ctctacatta gataaatgta taaataaggc ttgtaaagaa ttaggagtaa aagaagagga 19261 attaaattat gaaattatag aagagaaaca aggctttttc atgaaaaaga ctacaatatt 19321 agttaatgca gaagaaaaat taatgaacat gaaagaagat aaagttagta tagaagatag 19381 tgtagaagaa aatatagaac ataatgatga aaaaatagaa atagaaaagg agtcaatagc 19441 taaagaaagt atagcagcta atgatggaaa agtaaaaata gtaaaaaacc aagtaatagt 19501 tacaaatcct aaagagggtg gaaaaccagc agctatagct ccaggagata atataacaat 19561 actagtagat ggagacagaa taaaatctag aaaagaagtc tttgaagaaa ataaaataga 19621 agttattttt gaagaagtta aggctcatag agagttaaat gttaaaacgg atcaaaataa 19681 tatggaagct tatatttcag taatatataa gccagaaata aagtatggat cgaaagatat 19741 agaagataaa aattatttaa tactaaattc ttgtaaaata gaagaaaaag atcctcctta 19801 ttatactgaa gaagaaatta tagaagcttt gaaaaagatg ggcatagttt atggaatagt 19861 aaaagaaaat ctagagcagt gtacaaaaaa taactgtata gaattgttaa tagctaaagg 19921 aaaggaaact attaacgaag aggatgaaag tatagagatt aaatttacaa cagataatca 19981 agaattaaaa ttaacagagg ataaaacagg aaatgtagac tttaaaagta taggttccat 20041 agaatctgtg aagccaggag aagtattagc tgtaagaaaa aaaggtaaag agggacaaga 20101 tggaatcgat ataaaaggta tagtaaaaaa acataaacaa ggaaagaaaa ttatattaaa 20161 accaggtcaa ggaacagttt taagggatga agataccata gaagctgcta tggaaggaaa 20221 gccctgcgta aaatccaata tattttatgt atatcaagtc catgaagtta aaagtgatgt 20281 ggatataagt acagggaata taacatttgt aggggatgta gtagtgcaag gttctgtaaa 20341 ggaaggtatg aaagtagaag caggaaactc tgtagaaata aaaaaacacg tagagagatc 20401 agaaataatt tcaaaaagta atttgaatat agatggcaat ataataaact ctgatatata 20461 cggtggtgga gaagatactt taaaggtaat ggtgttaaat aaattagaaa aattaaagga 20521 tactcttaca gaactaattt ctgctgtaga agaaataaaa agatttaatt tacttggaga 20581 aggtaaaaaa gacggagaaa taataaagat tttaatagag aataagttta gaagtttgac 20641 caaaatatgt atagctataa tggctaatat aaatatgtgt agaagtgatg acgaagaaga 20701 tgaattagta ggaattataa ggaaaaaatt aataggatta ggtcctgttc atataaaaaa 20761 ttacagagaa ttagagcaaa tagtaaatct tatagaagaa aaaatgaaaa tgtgtaagga 20821 cggattatca ctgcctgtta atgttaatat tagttattgt caagattcta aaatacaaag 20881 tacaggagat atttatataa cgggtaaagg tgaatatata tcaaaattaa caagcaataa 20941 taatgtttac tttactaaag atggtagtgt agcaagaggt gggcacataa cagctaaaaa 21001 tgaaataagg tgtaaagagg taggaagtga agcaggagtt attactaaac tacaaatttt 21061 acaaaaggga gacatatatg tagatgtagc ctatcaaaat acagtattta ttataggtga 21121 tagagaatat ttattggaaa caccaagtaa agatatacat gcttatttag ataaaaaagg 21181 agagattacc gtagaaaagt ttgtattata ggaggttaat ttagtaatgg atataagtga 21241 agctaaagta ttaatattta aaataaatgg agaatactat gccgcagata tcatggaaat 21301 agaaagaata ctaggttatg aagaacctac aaatttaccg gatgcaccaa gttttgttca 21361 aggagttata aactatcagg gtaaaatatt acctgtagtt tctctttcta gaaaatttaa 21421 tttaaaagaa gaagaaatta aaaatgaagc taaaataata gtagttaaag aggaagaaaa 21481 taaattaggt ataatagttg atgtggtttc agaggttaaa gacgtaaaat tagataatat 21541 agaacagcca ccagaaattg tagcaggtat atccaaaaga tatataaaag gattaataaa 21601 aatagaaaat aaaataataa tatttttaaa tttatcaact atactaacag aagaagaaaa 21661 atcaatgata tagttgaggg agtaattata tggatataaa agagataaaa gtgggtattg 21721 cagatttaaa tgtaggtaaa aatcctgaca agataataac agtaggacta ggctcatgta 21781 taggaatagc attatatgat ggaataaaat gtgtaggagg gctttcacat ataatgctac 21841 cagatagcac tcaatttagt aaggttacaa accccatgaa gtttgcagat ttagctatac 21901 ctatattagt agaaaaaatg gaaaaattgg gagcaagaaa aaatggatta aaggctaaaa 21961 tatgtggcgg agcatctatg tttaattttt cggataaaag tatggtaatg gatataggaa 22021 atagaaatgg aaaagcagta aaggaaaaat taaaggaact atctataccg ttgcttgcag 22081 aagatatagg tggaaacaaa ggaagaacta tgatatttga tacctctaca ggaaaagtat 22141 atataaaaac tgtaggctta ggaactaaag aaatatag //