LOCUS NZ_DS981517 23519 bp DNA linear CON 30-MAR-2017 DEFINITION Clostridium sporogenes ATCC 15579 Scfld_02_0, whole genome shotgun sequence. ACCESSION NZ_DS981517 VERSION NZ_DS981517.1 KEYWORDS . SOURCE Clostridium sporogenes ATCC 15579 ORGANISM Clostridium sporogenes ATCC 15579 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT REFSEQ INFORMATION: The reference sequence was derived from DS981517. Clostridium sporogenes (GenBank Accession Number for 16S rDNA gene: DQ911258*) is a member of the Firmicutes division of the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from ATCC (ATCC 15579). We have collected 8.9X coverage in plasmid end reads and 454 reads. We have performed one round of automated sequence improvement(pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs.These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gutmicrobiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representativesof the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. tRNA genes were determined using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and Rfam v8.0. Gene names are generated at the contig level and may not necessarily reflect any known order or orientation between contigs. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in May 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. Clostridium sporogenes (GenBank Accession Number for 16S rDNA gene: DQ911258*) is a member of the Firmicutes division of the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from ATCC (ATCC 15579). We have collected 8.9X coverage in plasmid end reads and 454 reads. We have performed one round of automated sequence improvement(pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs.These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gutmicrobiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representativesof the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline set; GeneMarkS+ repeat_region ##antiSMASH-Data-START## Version :: 5.0.0alpha Run date :: 2018-12-14 14:15:44 NOTE: This is a single cluster extracted from a larger record! Orig. start :: 2605571 Orig. end :: 2629090 ##antiSMASH-Data-END## FEATURES Location/Qualifiers cluster 1..23519 /aStool="rule-based-clusters" /cluster_number="7" /contig_edge="False" /core_location="[2615571:2619090](+)" /cutoff="20000" /detection_rule="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /neighbourhood="10000" /parent_cluster_number="7" /product="porA" /tool="antismash" cluster_core 10001..13519 /aStool="rule-based-clusters" /cluster_number="7" /cutoff="20000" /detection_rule="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /neighbourhood="10000" /parent_cluster_number="7" /product="porA" supercluster 1..23519 /child_cluster="7" /contig_edge="False" /detection_rules="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /kind="single" /product="porA" /supercluster_number="7" /tool="antismash" region 1..23519 /contig_edge="False" /product="porA" /rules="(minimum(6, [Fer4, Fer4_10, Fer4_6, Fer4_7, PFOR_II, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III, PGM_PMM_IV, POR, POR_N, TPP_enzyme_C, Transketolase_C]) and POR and POR_N and PFOR_II)" /supercluster_numbers="7" /tool="antismash" gene 1217..1831 /locus_tag="CLOSPO_RS12115" /old_locus_tag="CLOSPO_03785" CDS 1217..1831 /codon_start=1 /inference="COORDINATES: protein motif:HMM:PF14282.4" /locus_tag="CLOSPO_RS12115" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03785" /product="hypothetical protein" /protein_id="WP_003487113.1" /transl_table=11 /translation="MKINSTSSAPMLKQVMKNTGKDCTKNMEAEQKEIDKEIQSLQQKK AALNKEIDNIKNDFAPPKPKEELIRPLKDQIQILDAQIQQKQIERTKKQNENIQEVSND SDNVKSNFTLEDTEDKINPTSNNGKNKSSLKLLKNFTTQTGVINEERIKNISKNPDDPN TSDSTKSITEKKRQKYFKNAIDAYNTIENSNSVNLGTNVLS" gene complement(2004..2591) /locus_tag="CLOSPO_RS12120" /old_locus_tag="CLOSPO_03786" CDS complement(2004..2591) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011099656.1" /locus_tag="CLOSPO_RS12120" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03786" /product="hypothetical protein" /protein_id="WP_003487114.1" /transl_table=11 /translation="MITREEAWNLLNEYNKEAFHIQHALTVEGTMKYFAEKLGYEDEAE FWSMVGLLHDLDFEIYPEQHCIKTQEIMRQQGLDEKLIRAAASHGYGLCLGELPKPEHQ MEKVLFAVDELTGLIGAAARMRPSKSVMDMELSSLKKKFKDKKFAAGCSRDVIKQGAEM LGWELNKLFEETILAMRSCEAYVNNEMEVYNN" gene 2828..3379 /locus_tag="CLOSPO_RS12125" /old_locus_tag="CLOSPO_03787" CDS 2828..3379 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003490333.1" /locus_tag="CLOSPO_RS12125" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03787" /product="XRE family transcriptional regulator" /protein_id="WP_003487117.1" /transl_table=11 /translation="MNINSIIAENLKTLRTERNLSLGQLAELSGISKVMLSQIEKGDTN PTINTLWKIAKGLKVPYTSLLEQKEHDTYVIKKRDIEAQFTEEGHYRVYCYYTSTPYRN FELFQIEIDEGCSYKSIGHSKKCQEYIMVLEGELTLEVNNETYKLTPDDSISFSASNQH VYINSGNGTLKATITNFYPA" gene complement(3471..4916) /locus_tag="CLOSPO_RS12130" /old_locus_tag="CLOSPO_03788" CDS complement(3471..4916) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253726.1" /locus_tag="CLOSPO_RS12130" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03788" /product="glycoside hydrolase family 1 protein" /protein_id="WP_003487120.1" /transl_table=11 /translation="MKTNFPENFLWGGAVAANQCEGAYNIDGKGLSVQDVAPKGIKAGP TLEPTQDNMKLIGIDFYHRYKEDIKLLGEMGFKVFRLSIAWSRIFPMGDEKEANEKGLQ FYDDVFDECLKYGIQPIVTLSHYETPLHLSKVYDGWKCREMIGFFNKYVCTVFNRYKNK VKYWLTFNEINSITHAPFLSGGIYTPLEELSKQEIYQAIHHELVASATAVKACHEIIPD AKIGCMILGMPVYPMTPSSEDMWETLNKERENFFFSDVQVRGYYPSYSKRFFKENNVKL DITHEDIEILKNTVDFISFSYYMSICESSDKSIKPGEGNIIGGIPNPYLEASEWGWQID PKGLRYYLNILYDRYQKPLFIVENGLGAVDELVELEDGTKIVLDDYRISYLRDHLIQVA EAIEDGVDLMGYTSWGCIDLVSFTTSELKKRYGFIYVDRNDDGTGTLKRYKKKSFYWYK EVISSNGDIEALCSNISIEKDKI" gene complement(5216..6943) /locus_tag="CLOSPO_RS12135" /old_locus_tag="CLOSPO_03789" CDS complement(5216..6943) /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) NAD_binding_8" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253721.1" /locus_tag="CLOSPO_RS12135" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03789" /product="amine oxidase" /protein_id="WP_003487122.1" /sec_met_domain="NAD_binding_8 (E-value: 2.8e-11, bitscore: 40.7, seeds: 167, tool: rule-based-clusters)" /transl_table=11 /translation="MEINEFIQPNNPTYEERDNMLKSALEEVGRIEDYNNIKELLGPQA DITKIISPGKGKGINVGIIGGGAAGLSSAFELRKLGFNITIFEEQKERVGGRIYTYYFD KDKKMYGELGAMRIPVSHGTTWHYIDTFGLETRPFVQENENGIIYIRNKRARNDPEGKS VMKNIYPEFNLSTFEKNISWQEILSYALESELDKLDTSTKKELLQIKKEYSPKIKELGA ISTRKVMAGEGLSEGAIELLSYLSPIVGYFYYGSYIENLQDQYIANDYYRYYIKGGLSK LPISFYNSLTSKNPKEYISISNSNLGKVKWMNGRTVTGIYKMDEKNKVRIKYREGKSLE TYCEDFDYIICAIPFSSLRSVEIYPMFTPEKMQAIKELSYEAAQKTLFLCNNSFWEEGN KNERIIGGSSRTDLIISSIWYPNNHLIKNIEISKNKKKHKKHKNYKSWSNPGVLLASYN LNLDAVRLGNIDEKIRVELIKRQVEKVHGLPKGYLNSIVESYKTIEWDHEQGFYGGISH YREEQQNLFAYASEQPEYDERVYFAGEHISQTHAWIQGALSTGMKAANRIAEHYKYKA" gene complement(7353..8828) /locus_tag="CLOSPO_RS12140" /old_locus_tag="CLOSPO_03790" CDS complement(7353..8828) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253720.1" /locus_tag="CLOSPO_RS12140" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03790" /product="YdiU family protein" /protein_id="WP_003487124.1" /transl_table=11 /translation="MKERKVIIKTGLNLENSYASLPEIFFTRQSPSHVPSPKLAVLNYP LITSLGLNAKVLQSADGVDILAGNKTPEEAIPIAQAYAGHQFGHFTMLGDGRALLIGEH ITPLGDRFDIQLKGSGKTPYSRGGDGKAALGPMLREYIISEAMNALGIPTTRSLAVVTT GESITRETELPGAILTRVAASHIRVGTFEYVSRWGTVEALRALADYTLKRHFKEGNDKE NPYLFLLQEIIKKQAELIAKWQLVGFIHGVMNTDNMAISGETIDYGPCAFMDAYDPETV FSSIDIYGRYAYGNQPNIAAWNLARLAETLLPLLHINPNEAIKIAENAVSDFTKLYKNN WLSGMRAKLGIFNEELQDEYLIEDLLSIMHKYGADYTNTFHALTFDNIEDTALFGKVEF DKWYKLWQERLTRQEESKLSSKQLMKSGNPSVIPRNHRVEEALEAAVKEGDYSVMEKLL DALSKPYDHSKEQDYYSKLPGPSTCPYQTYCGT" gene complement(9149..9610) /locus_tag="CLOSPO_RS12145" /old_locus_tag="CLOSPO_03791" CDS complement(9149..9610) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003357140.1" /locus_tag="CLOSPO_RS12145" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03791" /product="DUF1905 domain-containing protein" /protein_id="WP_003487126.1" /transl_table=11 /translation="MKTYTFEAEIKKIEGKDATYIEIPFDVEKEFSAKRVKVLVKFENE QYRGSIVNMGLACYIIGITKEIRNKIDKTYGDIIKVELQKDEEERIVIVPEEFKVKLSN NKVANDFYESLSYSYKRKYIQWLTSAKKEETKVKRMEEAIVKLENKIKI" gene 10001..13519 /locus_tag="CLOSPO_RS12150" /old_locus_tag="CLOSPO_03792" CDS 10001..13519 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) porA: POR_N" /gene_functions="biosynthetic (rule-based-clusters) porA: TPP_enzyme_C" /gene_functions="biosynthetic (rule-based-clusters) porA: POR" /gene_functions="biosynthetic (rule-based-clusters) porA: Fer4_10" /gene_functions="biosynthetic (rule-based-clusters) porA: PFOR_II" /gene_functions="biosynthetic (rule-based-clusters) porA: Fer4_7" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_9" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_16" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253717.1" /locus_tag="CLOSPO_RS12150" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03792" /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase" /protein_id="WP_003487127.1" /sec_met_domain="PFOR_II (E-value: 2.4e-17, bitscore: 60.3, seeds: 27, tool: rule-based-clusters)" /sec_met_domain="POR_N (E-value: 3e-80, bitscore: 266.5, seeds: 31, tool: rule-based-clusters)" /sec_met_domain="POR (E-value: 1.6e-33, bitscore: 113.4, seeds: 86, tool: rule-based-clusters)" /sec_met_domain="TPP_enzyme_C (E-value: 4.5e-10, bitscore: 36.7, seeds: 323, tool: rule-based-clusters)" /sec_met_domain="Fer4_10 (E-value: 4.2e-08, bitscore: 30.4, seeds: 59, tool: rule-based-clusters)" /sec_met_domain="Fer4_7 (E-value: 4.1e-10, bitscore: 37.4, seeds: 208, tool: rule-based-clusters)" /sec_met_domain="Fer4_9 (E-value: 4.2e-07, bitscore: 27.3, seeds: 61, tool: rule-based-clusters)" /sec_met_domain="Fer4_16 (E-value: 1.4e-10, bitscore: 39.2, seeds: 227, tool: rule-based-clusters)" /transl_table=11 /translation="MAKIMKTMDGNQAAAEASYAFTEVAAIYPITPSTPMAEGVDEWSA HGKKNIFGQSVKVVEMQSEAGASGTVHGSLVAGALTTTYTASQGLLLMIPNLYKIAGEL LPGVFHVSARALATHALSIFGDHQDVMATRQTGVALLAASNVQEVMDLANIAHLSAIKS KVPFLHFFDGFRTSHEYQKIETIDYNDVAKLVDYTALKEFKSRALNPEHPTLKGTAQNP DIYFQGREASNKFYKEIPNIVENYMREIEKITGRSYHPFDYYGDPNAENIIVAMGSVCD TIEETVDYLINKGEKVGVIKIHLYRPFCPTYFMKSLPKTVKKIAVLDRTKEPGSIGEPI YLDICKVFYNTKDKPIIVGGRYGLGSKDTRPSQIISVFDNLNQDNPKDGFTIGITDDVT NTSLPEKDVVDTTPEGTISCKFWGLGSDGTVGANKTAVKIIGDNTDLYAQAYFSYDSKK SGGSTISHLRFGKDKIKSPYLIYNADYIACHNKSFIYNFDVLKGLKKNGTFVLNCPWDE SELEEKLPASMKKYIAENNINFYIIDGISIAQNIGLGGRINMVMQSAFFKLANVIPVNE AVELLKNSVEKTYGKKGEKIVEMNKAAIDAGIDAVHKVNIPSSWKNVETELASIKKEPD FIKKIQRPISRHEGDELPVSAFNGMADGTFPLGTTSYEKRGIAVMIPEWQIDKCIQCNQ CSYICPHSVIRAYLLDKDEKEKAPDTFETKKAAGKGLEDLEYRIQISSLDCTGCGNCAD VCPAPGKALIMKNAEEQIEMQSENWEFGLNITTKENLMDPKTLKGSQFIRPLLEFHGAC PGCGETPYIKLLTQLFGDRMMIANATGCSSIWGGSAPSVPYTTNSQGKGPSWGNSLFED NAEYGYGMYLAVKQVRERLANLMQEALNMEINQNLKETFKQWLDSMDDGEGSKIATAKI LETISNENYQNNPILNEIMEKKDYLIKKSHWMIGGDGWAYDIGFGGLDHVLASGDDVNI FVMDTEIYSNTGGQCSKSTQTGAIAKFAAAGKKIKKKDLGLMAMSYGYVYVAQIAMGAN MNHTIKTIAEAEAYKGPSLIIAYAPCISHGIKTGMGTSIAEEKKAVQSGYWHLYRYNPM LKEEDKNPFILDSKEPTKPFKEFIQGEVRYSSLMNIFPDIAAKMSDMAEQHARERYDNY RHLADK" gene complement(13559..13726) /locus_tag="CLOSPO_RS12155" CDS complement(13559..13726) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001387101.1" /locus_tag="CLOSPO_RS12155" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /product="DUF3787 domain-containing protein" /protein_id="WP_061329843.1" /transl_table=11 /translation="MDKNNNKESFMAMPIEKHDTAAWANIESMKPICNVSIPSEVEVRN AKEWVDTNQK" gene complement(14083..15969) /locus_tag="CLOSPO_RS12160" /old_locus_tag="CLOSPO_03794" CDS complement(14083..15969) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253715.1" /locus_tag="CLOSPO_RS12160" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03794" /product="glycerophosphoryl diester phosphodiesterase" /protein_id="WP_003487132.1" /transl_table=11 /translation="MTTRMETKHNKPISTLKMIRKNFTYFKKSFFINIGFESIYKVLTS FIFLPFITMVLNYFMHLRGMKMMANNQILSFIGSIPGILTILILGILSFLFIFIEYAGL IVISQETYFDKKISAKASFFTAMKSIRGIIKIGSIPIVIYVLFIIPFVASSMGLSSSLL PKIEIPAFIMDVVYKTPKWISLYAIVFFILLYFLIRWIFSLHVVVLEGKDFFSALKRSS QLVKGSFLKIWTTMFFWNLSIFAIVAVVVILVGIGMVLLNEVLKGNVKLFVTILSFVTT MGGILVMIATLFIYPLTIMLITQLYYRQAKMKDGVEPTPMIITVTPSKINWQNLFNRHK KKLIAVSVLGIMIFTWLSYIYFITYEFDANNNLVTVTAHRGDAVNAPDNTMSSLLSAID NKADYSELDVQETKDGVVVLTHDTNLKNNTGVNKNIWELTFDEVEKLDVGSHFSPKFAG EKIPTLDSIMKSANKNIKLNIEIKLNGHDQKLEERVVKLIEDNNFVDQCVVTSLDYNVL QKVKNLNSRIKVGYIVFAGMGDVTALNVDFLSVEEAVATPEFIEKVHKNNKSIHVWTIN DPEKMEKFIELQVDSIITDNSKEVIRLIQEKKDKQLRNEIDYIYKIVALFND" gene <16235..16327 /locus_tag="CLOSPO_RS19420" /pseudo="" CDS <16235..16327 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017826477.1" /locus_tag="CLOSPO_RS19420" /note="incomplete; partial in the middle of a contig; missing start; Derived by automated computational analysis using gene prediction method: Protein Homology." /product="hypothetical protein" /pseudo="" /transl_table=11 /translation="FIHHLEINTKFLGLDIEIKSKEKSAPSSQD" gene complement(16397..17140) /locus_tag="CLOSPO_RS12165" /old_locus_tag="CLOSPO_03795" CDS complement(16397..17140) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253714.1" /locus_tag="CLOSPO_RS12165" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03795" /product="ABC transporter permease" /protein_id="WP_003487134.1" /transl_table=11 /translation="MFKVLKNEIIKMFSGKKFYVLSGILIISIVIMSFLGKSHPQDGMN ANNFVIETLFGMLMKPMVPIFMVLVIAETLTEDYIHGTMKFSLMTPIKKSDFIIGKFLF IALYALIFLIVGFVGSYIVGAIAFGLGNNGDNLNLFINNIKVYASIILPLLSFSAVVSF IAMIINNSGAMIGLGIGIDVIMMMIDSQMKNIMYYTFSGGMYAYRIVQNLNAHNILMLS LTACIYITVFLVLSIVVVERKDIVL" gene complement(17133..18056) /locus_tag="CLOSPO_RS12170" /old_locus_tag="CLOSPO_03796" CDS complement(17133..18056) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253713.1" /locus_tag="CLOSPO_RS12170" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03796" /product="ABC transporter ATP-binding protein" /protein_id="WP_003487136.1" /transl_table=11 /translation="MKKKCLLSTHNLTKEYKNKKAVNNLNITVYEGDVYGFLGPNGAGK STTIKSIMGLIKPTSGKVIINGYDVEKDSEKAIEKIGAMVEAPSFYGGVSGYKNLILMA NLYGVPKKRVDEVLQMVRLTDVAHKNVSKYSLGMKQRLGIARAFLNNPNIVILDEPTNG LDPQGIKDIRGLIQDLSKKYHVTFLISSHILSEIQAVCNRIGIIEKGSLKVEGYVEDLL NKDEEVIEIYTKEKEKTSRLLKNMNISSKIQCFEGGIRIKIKKGNFQNINKLLISNDVN VENLHCSETSLEDYFFNVMEGDKKYV" gene complement(18305..19282) /locus_tag="CLOSPO_RS12175" /old_locus_tag="CLOSPO_03797" CDS complement(18305..19282) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011988062.1" /locus_tag="CLOSPO_RS12175" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03797" /product="GHKL domain-containing protein" /protein_id="WP_042385591.1" /transl_table=11 /translation="MLPIIVFASLIIKRTESKKRRNKEISSSQIRSRMILMISTLLTVM IASIYIFTCTSIINNQKIDFIMGNFVPEALPLISIILTSIIVYYYDKSVEYRVELRREI EEKNEIEEYAHIIEEMYSETRRFKHDYINMLSPLKEYIDNEDIEGLSEFFYNNVIDMDK NIKWSNSNIDKLKYIKVSGLKAILSTKLIKASSMNIDINVHIVEDIDYIHMNIMDLCRI IGILMDNSIEAAGECEAPKICIAVANKNDYVVIAVHNNFFGDKPLIHKIYKEGFSTKGK GRGLGLYTVKNIIDTKYENVFLNTSIEENMFIQEIWIKYIEKTS" gene complement(19657..20376) /locus_tag="CLOSPO_RS12180" /old_locus_tag="CLOSPO_03798" CDS complement(19657..20376) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253711.1" /locus_tag="CLOSPO_RS12180" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03798" /product="DNA-binding response regulator" /protein_id="WP_003487138.1" /transl_table=11 /translation="MLRVIICEDNEVHRDKLKQIVENTILREKLDLDVVLATESPEEII SYIKENRNTGIYLLDVDLKNDINGIKLGEKIRELDPLGYIIFTTTHLEMSYLAFKYKVE AMDYVIKDDDDFKERVNSCIIKAYNRYHKQEHEEEYISIDTDTRIINIRSSDILFIETT GTPHKIRIHEETRQIELYANLKDMQKKLPSNFYRCHKSYIVNKDKIEEIDKKHGKIIME NEEECYVSFRYMRGLLV" gene 20647..20859 /locus_tag="CLOSPO_RS12185" /old_locus_tag="CLOSPO_03799" CDS 20647..20859 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014520357.1" /locus_tag="CLOSPO_RS12185" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03799" /product="hypothetical protein" /protein_id="WP_003487139.1" /transl_table=11 /translation="MGTKSFIITYGLPVNSESNVFDPNKLLLVVFNPVTISDLVIKPRS KAKTSHELILYFEDRAFLLFGNTVQ" gene complement(20937..22007) /locus_tag="CLOSPO_RS12190" /old_locus_tag="CLOSPO_03800" CDS complement(20937..22007) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253710.1" /locus_tag="CLOSPO_RS12190" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03800" /product="tRNA 2-thiouridine(34) synthase MnmA" /protein_id="WP_042385322.1" /transl_table=11 /translation="MKKKVLVGMSGGVDSSVAAYLLKEQGYEVIGVTMQIWQDDEEFIE KEGGCCSLSAVADARRVANKIGIPFYVMNFKDAFKRNVIDYFVDEYMEGRTPNPCIACN KFIKFSSFLDKAMAMGIDYVATGHYAIIEKHNDRYIIKKSEDDKKDQTYALYNLTQFQL ERTLMPCGQYKKSKIREIAKEIGLRVHNKKDSEEICFIPDNDHGRYIKNRFPNKVREGN FVDKQGNILGTHKGIVYYTIGQRKGLGIAFGKPMYVVDINPFRNEVVLGDLEDLLNTEL IAKDTNYIPFDTLKEPMEVEAKIRYSQIPSKAIITTVEDGRVRVNFHEKQRAITKGQSV VFYKDDLLIGGGIIEK" gene complement(22122..22250) /locus_tag="CLOSPO_RS12195" /old_locus_tag="CLOSPO_03801" CDS complement(22122..22250) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001387094.1" /locus_tag="CLOSPO_RS12195" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03801" /product="hypothetical protein" /protein_id="WP_003356573.1" /transl_table=11 /translation="MNILKRLIKKLEKANEKEFENKKLDCCDLNKTNMVNNKPKSK" gene complement(22405..23205) /locus_tag="CLOSPO_RS12200" /old_locus_tag="CLOSPO_03802" CDS complement(22405..23205) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001253709.1" /locus_tag="CLOSPO_RS12200" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CLOSPO_03802" /product="TIGR00268 family protein" /protein_id="WP_003487144.1" /transl_table=11 /translation="MNYKYNNLINYLKDLGSVAVAFSGGVDSTLLLKAAKEALGDNVIS ITVVSPYIPKWEIEEAKELANNIGIKSYFLEVPMLEEIRFNPEDRCYICKKGVFSKIKE LAKEKGVKYIVDGTNLDDTKDYRPGMRALKELDVKSPLLENSINKEKIRTLSKELGLQT WNKPAYACLLSRIPYNQEIKEEDLSRIEKAEVYMMELGFRAVRVRSHGDLARIEVPKKE RVKLFNEDILDKVSEKFKKLGFKYVTVDTEGYKMGSLNTEINKN" ORIGIN 1 gtaaatgatc tgctttggtt tatactttta tgttatttgt gttatggaaa tattcttata 61 aaactttatg ctgctattgt tccagacgca attttattgc ttatttatat tactttttta 121 agctttgatt ttcatatttc ttcctacata aatagtttaa atataggctc tggaagagac 181 ctttttcttc tttgcctaat aaaccttggt cgtgaactta taaatttagc tctatttttt 241 atggttttga aaaatatttg caaattttta aatttcaaag atagaactgt aaatacctat 301 caaagcttat atttacttat tccatgcttg gcaacttata gtttaatatt gattttctat 361 tttgttcaag ctattcatgt tgacaataaa gactactatt tattttccat ttttcctgga 421 atttattctg ctgtgcctct ggtaagtatt tctttattag tttctatact tatcaccgcc 481 tacaccttta agaaaatgct tgaaggagaa gaaattcagc aaaaaaactt attaatggaa 541 caacaattta aattgcaaat tgatcacagt aaaaatcttg agggtgttta tagtggtatt 601 cgcagaataa tgcatgatat gaacaaccat cttatttgct taaaaaatct agctaaaagt 661 aaaaacattg aagagataaa taactatcta gatactcttg gtcaaacaat aaataagctg 721 gattgtaaga taaaaacagg caatgctgct tccgatgcag ttataaacga aaaatataat 781 attgctaaac tggaaggtat tgaattcagc tgtgattttc taatacctga gggtctgaca 841 ctaagttctg tagatttatg tatcattttg ggcaatgctt tagataatgc actggaagct 901 tgtatgagga ttaaaaatgg aaatattcac aaagaaattt ctgttaattc ttatattaga 961 gatctatacc taattattga ggtttcaaac agtgctacag ataaaattca gtataataag 1021 aataaaattg tgactaaaaa aaatgataag atcaaccatg gtataggaat ttctaatata 1081 gaaatgatag tagaaaaata taatggaatt ttagatatta tagaaggaaa aaataaattc 1141 gttttaaatc ttatgcttaa agtaaaagaa acttaataat tccttatata ccgactgtaa 1201 aaaggagatg ttttaaatga aaattaattc aacttcttct gctccaatgt taaaacaagt 1261 tatgaaaaat accggtaaag attgcacaaa aaacatggaa gcagagcaaa aagaaattga 1321 taaagaaatc caaagtcttc agcaaaagaa ggctgcttta aacaaagaaa ttgataatat 1381 aaaaaatgac tttgcaccac ccaaacctaa ggaggaattg atcagacccc taaaagatca 1441 aattcagatt ttagatgctc aaattcagca aaagcaaata gaaagaacaa aaaaacaaaa 1501 tgaaaatata caggaagttt caaacgactc tgataatgtg aaatcaaatt ttactctaga 1561 agatactgaa gataagatta accctacttc taataatgga aaaaataaat cttctcttaa 1621 actattaaaa aacttcacta ctcaaactgg agttataaat gaggagagga taaaaaatat 1681 ttcaaaaaat cctgatgacc ctaatacttc tgactctacg aaatccatta ctgaaaaaaa 1741 gagacagaaa tattttaaaa atgctattga tgcatataat acaatagaaa atagtaattc 1801 agttaatttg ggaacaaatg tgttatccta aataaaatac agttgccata cacaaactcc 1861 atactccaaa atataaggtt atatatagat tttaatttaa gcaaatctat atataacctc 1921 tttttagctt tatataaata tatatttaaa atttaattta gactgtattt tttagctaaa 1981 cttctttagt atgaatatat acactaatta ttatatactt ccatttcatt attaacatat 2041 gcttcacaag aacgcatagc taaaattgtt tcttcaaaca atttatttaa ctcccatcca 2101 agcatctctg caccttgctt aattacatcc cgtgaacatc ccgctgcaaa tttcttatcc 2161 ttaaattttt tcttaaggct tgaaagttcc atatccatga cactcttaga tggacgcatt 2221 cttgcagctg caccaataag tcctgtaagt tcatctactg caaataatac tttttccatc 2281 tgatgctctg gtttaggaag ttctcctaag caaagtccat atccatgact agctgctgcc 2341 cttattaatt tttcatcaag accttgctgt ctcataattt cttgagtttt gatgcaatgc 2401 tgttctggat agatttcaaa atctaaatca tgaagcaacc caaccataga ccaaaattct 2461 gcttcatctt catatcccaa tttttctgca aaatatttca tagttccttc aactgttaaa 2521 gcatgttgaa tatggaatgc ctctttattg tattcattaa gtaaattcca cgcttcttcc 2581 cttgttatca taataatatc ctcctttaaa aattacaaaa tatgaataaa aatgaatctt 2641 cactaaaata cttaagttcc ctatccttag atgcaaaaac aaaacttttc tatttattct 2701 catttctctt gtttcattta agtaattatt atataatatt ttacgaacgt taaatatatt 2761 tagcgttagt tttattttac ttttgttagt tataattgtc aataacatta taggaggtta 2821 taccctaatg aatataaatt ctattattgc tgaaaacctg aaaacacttc gtactgaaag 2881 aaatttaagt ctaggtcaac ttgctgaatt atctggtatt agtaaagtca tgctatctca 2941 aattgaaaaa ggtgacacta atcctactat aaatacttta tggaaaatag ctaaaggatt 3001 gaaagtgcct tatacatcat tacttgagca aaaagaacat gatacctatg ttataaagaa 3061 aagggatatt gaagctcaat ttactgaaga agggcattat cgagtatatt gttactatac 3121 tagtacaccc tatcgtaatt ttgaactttt tcaaattgaa attgatgaag gttgttcata 3181 taagtctata ggtcattcta aaaaatgcca agaatatatt atggtgttag aaggtgaatt 3241 aacattagaa gtaaacaatg aaacttataa actaactcct gatgattcaa taagtttttc 3301 tgcatctaat caacatgtat atatcaatag tggaaatgga acattaaaag ctacaataac 3361 aaatttttat cctgcctaat tattaggcag gatattttat gtaggtcata taaatttaat 3421 gaaacactaa agtaataata aaaactaaaa attatttata ctatctcaaa ttatatttta 3481 tctttttcaa tactaatatt tgaacatagt gcttcaatat ctccattact tgaaattact 3541 tctttatacc agtaaaagct ttttttctta taacgtttta atgtccctgt tccatcatca 3601 ttacgatcta cataaataaa tccataacgt ttctttaact cagaagtagt aaaactaact 3661 aaatcaatgc acccccaaga agtatatccc attaagtcta ctccatcttc aattgcttct 3721 gctacctgaa ttaaatgatc tcttaaatat gaaattctat aatcatctaa tactattttt 3781 gtcccatctt ctagctcaac taattcatct acagctccta atccattttc aactataaat 3841 aatggttttt gataacgatc atataatata ttaagatagt atcttaatcc tttaggatca 3901 atttgccatc cccattcgct tgcttctaaa taagggttag gtattcctcc aataatattt 3961 ccttcacctg gttttataga tttatcacta gactcacaaa tgctcatata ataactaaat 4021 gaaataaaat ctacagtatt ttttaatatt tctatatctt catgtgtaat atctaactta 4081 acattatttt ctttaaaaaa tctttttgaa tacgatggat aataaccacg tacttgaaca 4141 tctgaaaaga aaaaattttc tctttcttta ttaagagtct cccacatatc ttctgatgat 4201 ggtgtcattg gatatacagg catacctaaa atcatgcatc ctattttagc atcaggaata 4261 atttcatgac atgcttttac tgctgttgca ctagctacta attcatgatg aatagcttga 4321 tatatttctt gtttgcttaa ttcttctagt ggtgtataaa ttccaccact taaaaatggc 4381 gcatgagtaa ttgaattaat ttcattaaag gttaaccagt attttacttt atttttatat 4441 cgattaaaaa ctgtacaaac atacttatta aaaaatccaa tcatctcacg acatttccat 4501 ccatcgtaaa ctttagataa atgcaatggt gtttcataat gggataatgt tactattggc 4561 tgtattccat actttaagca ctcatcaaaa acatcatcat aaaattgtag tcccttctca 4621 tttgcttctt tttcatcccc cattggaaat attcttgacc acgcaattga taacctaaaa 4681 actttaaatc ccatttctcc caaaagctta atatcttctt tatatctatg ataaaaatct 4741 atacctataa gtttcatatt atcttgtgta ggctctaatg taggaccagc ttttattccc 4801 tttggcgcta catcttgcac gcttaatcct ttaccatcaa tattataagc tccttcacat 4861 tggttagccg caactgcacc accccacaaa aaattttctg gaaaatttgt tttcataatt 4921 aatcccccat attttcatta ttttttatta tatcaatagt ttcttcatct aatagtagat 4981 tatacaaagc cgttatcttt actgtacttt aattttataa tcaacttatg ataaaatcta 5041 tatcataaat gaatggattt ctcctacatt agataccttc ttgacaatgt gcaagaaaga 5101 aaatggtaac ttattcattt tcatatctaa aagatatttt taatataaat acatattatc 5161 actaaatata agcataaata aagaagcata ttaaataaaa tatacttctt tattcctaag 5221 ctttatattt atagtgttct gctattctat tagctgcctt cataccagta cttaaagccc 5281 cttgaatcca tgcatgtgtt tgtgatatat gctcacccgc aaaatatact ctttcatcat 5341 actctggctg ttctgatgca tatgcaaaaa gattctgttg ttcttcccta taatgactaa 5401 ttcctccata aaaaccttgt tcgtgatccc attctattgt tttataggat tccactattg 5461 aattaagata tcctttaggt aaaccatgta ccttctcaac ctgtctcttt attaattcta 5521 ctcttatctt ttcatctata ttacctaacc gtacagcatc aaggtttaga ttgtaggatg 5581 ctaaaagcac tcccggatta ctccatgatt tataattttt atgtttttta tgcttctttt 5641 tatttttact aatctctata tttttgataa ggtgattatt tggataccat atacttgaga 5701 ttattaaatc tgtcctagaa ctgccaccta ttattctctc atttttattt ccttcttccc 5761 aaaaactatt attacataga aataatgttt tttgagctgc ttcatagctt aattctttaa 5821 ttgcttgcat tttttctgga gtaaacatag gatatatttc tacacttcga aggcttgaaa 5881 atggtattgc acaaattata taatcaaaat cttcacaata tgtttctaaa gattttcctt 5941 ctctatattt aattctaacc ttattttttt catccatctt ataaatccca gttactgttc 6001 gtccattcat ccattttact tttcctaaat tagaatttga tatgcttata tactctttag 6061 gatttttaga tgttaacgaa ttataaaaag atataggtaa tttagataac cctcctttta 6121 tataatatct ataataatca tttgcaatat attgatcttg taaattttca atataactac 6181 catagtaaaa ataacctaca attggagaaa gataggataa taattctata gccccctcac 6241 ttaaaccttc acctgccata acctttcttg tacttatagc tcctaattcc tttatctttg 6301 gagagtactc tttttttatc tgtaataatt ctttttttgt actagtatct aacttatcca 6361 attcactttc taatgcataa ctcaaaattt cttgccatga tatatttttt tcaaaggtag 6421 acaaattaaa ttctggatat atgttcttca ttacactttt accttctgga tcattccttg 6481 cacgtttatt tctgatatat ataattccat tttcattttc ttgaacaaaa ggtctagttt 6541 ctaacccaaa agtatctata taatgccaag ttgttccatg acttacaggt atacgcatag 6601 ctccaagttc accatacatt tttttatctt tatcaaaata atacgtatat atccttcctc 6661 caacacgttc tttttgttct tcaaaaatag ttatattaaa acccagtttt ctaagttcaa 6721 aggcagagga taatcctgca gcacctccac ctattatccc aacattaatt cccttacctt 6781 tacctgggga aataattttt gtaatatctg cttgaggccc aagtaattct tttatattat 6841 tgtagtcttc tattctcccc acttcttcta atgctgactt tagcatatta tctctttcct 6901 cataagttgg attattaggc tgtataaact catttatctc caaatagttc aaccccttaa 6961 gttagtctta acatcaataa cttagtattc attatctaat agtattctaa atagcatttt 7021 tttatgtcat cacattatca atgtaattat tgtaatattt aatttttaca ataatcataa 7081 gaataatctt acaccaatta aaaaatatgg aatttgctgt aaataaaaac aaccggcaca 7141 agttcagctg ttcttttaaa atctaatcta actaatatta gttaatatgt tattattttt 7201 atttatttcg gttgtccaca aatacagttc atcaaaagtt tggactttcc tatatacaat 7261 ataattaata tattttcaag ttcagagttc tattatattt ttatacaaaa agagctagta 7321 aaacttaata tattttacta gctccttttg tattaggttc cacaataggt ttgatacgga 7381 caagttgatg gtccaggcaa tttagagtag taatcttgtt ctttagagtg atcatatggt 7441 tttgaaagag catctaaaag tttctccatc acactatagt ctccttcttt caccgcagcc 7501 tctagtgctt cttctaccct gtgattacgt gggattactg atggattacc acttttcata 7561 agctgttttg aagaaagctt tgattcttct tgtcttgtta gcctctcctg ccatagctta 7621 taccacttat caaattccac tttaccaaac agtgccgtat cctctatatt atcaaaagtc 7681 aatgcgtgga atgtattggt ataatctgca ccatacttat gcattatact tagaagatct 7741 tcaattaggt attcatcctg aagctcttcg ttaaatatcc caagttttgc cctcattcca 7801 gaaagccaat tattcttata caacttagta aaatctgaaa ccgcattttc tgctatcttt 7861 atggcctcat ttggattaat atgcaacaat ggtaacagag tttcagcaag ccttgcaaga 7921 ttccatgcag caatattagg ttgattacca taggcatatc taccataaat atcaatggaa 7981 ctaaatacag tttcagggtc atatgcatcc atgaatgcac aaggaccata atcaattgtt 8041 tctccactaa ttgccatatt atcggtattc attaccccat gaataaaacc aaccagttgc 8101 cattttgcaa tgagctcagc ctgcttcttg attatctctt gaagtaagaa cagataagga 8161 ttctctttat cattcccttc tttaaaatgt cttttcaaag tataatcagc tagagctcta 8221 agcgcttcaa ctgtacccca tcttgaaacg tattcaaatg tgccaacacg tatatgactg 8281 gcagctacac gagttaatat tgcaccgggt agctcagttt cccgcgttat tgactcacct 8341 gttgtaacca cagctaaact acgggtggtt ggaattccta atgcattcat tgcctcactt 8401 ataatgtatt ctcttaacat tggacctagc gctgcttttc catcgcctcc tcgtgaatat 8461 ggagttttac ctgaaccctt aagctgaata tcaaatctat cacctagtgg agtgatgtgt 8521 tcccctatta gcagagctct tccatcaccc aacatagtaa aatgaccaaa ttgatgtcct 8581 gcatatgctt gagcaatagg tatagcctct tctggagtct tgtttcctgc aagtatatct 8641 actccatcag cactttgtaa tactttggca tttagtccca aggatgttat taacggataa 8701 ttcaatacag ctaattttgg tgaaggtaca tggctcgggc tttgtctagt aaaaaatatt 8761 tctggaaggc tagcatagct attttctaaa ttcaagcctg tttttattat tacttttctt 8821 tctttcattg tatctccttt tcttctctta ttttttatat aaattcttta catgaaacaa 8881 aatcgtagtt tctattactt ttcagatata aacttctatg ataactaaac tctttttagt 8941 ttatacttct aaaaatttat tattcccgct acatttctct ttatctccta actatttaat 9001 aacttaatct atgaatttat attttatctc tctataatgc caatatataa taatattaat 9061 taaatattta atataaaatt catgtaaaat ttaaaataac ccaatgtaag taataatgtt 9121 attacttaca ttgggttata aatgaaaatt atattttaat tttattctct aatttaacta 9181 tagcttcttc cattcttttt actttagtct cttctttttt agctgatgtt agccattgaa 9241 tatatttacg tttataagag tatgataaag attcataaaa atcatttgct accttattat 9301 tagaaagttt aactttaaat tcttctggta caataactat ccgttcttcc tcatcctttt 9361 gtagttcaac ttttataata tctccataag ttttatcaat cttatttctt atctccttag 9421 tgattccaat tatataacaa gctaatccca tatttacaat tgaacctcta tattgttcat 9481 tttcgaattt tactaatacc ttaactcttt tggctgaaaa ctctttttca acatcgaaag 9541 gtatctctat atatgtagca tcttttcctt caattttttt tatctctgct tcaaaagtat 9601 aagttttcat ataagtttta atcctcctat gtaaaaatga tacatttctt tataaataga 9661 ctatatatta tttaaaatta tattacttat tactaactca tcctttgcaa tcagattata 9721 acataaatca atgacatttt taacattatt ggcttgtttc cttatatttc atcatattcc 9781 atttatttgt cctccccaca gatactcttt accgtatcat aaaaaaagat ggtaagtata 9841 aaaaatcttt ttaagctatt aagcagtaaa gttaaaggaa atttaatgac ttttctttta 9901 gtcctctatc catatttatg ttacatgtat ttataatact ttactagtaa ataataaaca 9961 tgtcttatta aattaaaatc ttaggaggga gaaatatttt atggcaaaaa ttatgaaaac 10021 aatggatgga aaccaagcag ctgctgaggc atcctatgct tttactgaag ttgcagctat 10081 ctatccaatt acaccttcca cccctatggc agaaggtgta gacgagtggt ccgctcatgg 10141 gaaaaaaaat atatttggtc agtctgtaaa agttgttgaa atgcaatctg aagctggggc 10201 ttctggaact gttcatggct ccttagttgc tggtgcactc acaactacat acactgcttc 10261 tcaaggtttg ttgttaatga ttcccaatct atataaaata gctggtgaac tattacctgg 10321 tgtatttcat gtgtctgcta gagccttagc cactcatgct ttatcaattt ttggtgatca 10381 tcaagatgta atggctacaa gacagactgg tgtagcactt ttagcagctt caaatgttca 10441 agaagtaatg gacttagcaa acatagcaca tctatccgct attaaatcta aagttccatt 10501 cttacacttt tttgatggct ttagaacttc tcacgaatac caaaaaattg aaactattga 10561 ttataatgat gtagctaaat tagtagatta tactgctcta aaagaattta aatctagggc 10621 attaaatcca gaacatccta ctcttaaagg aactgctcaa aatccagata tttattttca 10681 gggaagagaa gcatcaaata agttctataa agaaatacct aatattgttg aaaactacat 10741 gcgggaaata gaaaaaatta caggaagatc ctatcatcct tttgattatt atggagatcc 10801 taatgcagaa aatattatag ttgctatggg ttccgtatgt gatactatag aagaaacagt 10861 agattattta ataaataaag gagaaaaagt aggcgttata aaaattcact tatatcgccc 10921 attctgtcca acatatttta tgaaatctct acctaaaact gtaaaaaaaa ttgctgttct 10981 tgatcgtaca aaagagcctg gttcaatagg agagcctata tatcttgata tatgtaaagt 11041 attttataat acaaaggata agcctattat tgtaggtggt agatatggtt taggatctaa 11101 agatacaaga ccttctcaaa ttatttcagt atttgataat ttaaatcaag ataaccctaa 11161 ggatggattc acaataggaa ttactgatga tgttaccaat acatcattac ctgaaaagga 11221 tgtagttgac acaactcctg agggtactat aagttgtaag ttctggggat taggttctga 11281 tggaacagta ggtgcaaata aaactgctgt aaaaattatt ggagataata cagatttata 11341 tgctcaggca tacttttctt atgacagtaa aaaatctggt ggtagcacca tttcccactt 11401 aagatttgga aaggataaaa taaaatcacc ttatttaatt tataatgcag attatattgc 11461 ttgccataac aaatccttta tatacaattt tgatgtccta aagggattaa agaaaaatgg 11521 cacatttgta ttaaattgtc cttgggatga atcagaacta gaagaaaaac taccagcttc 11581 tatgaaaaaa tatatagcag aaaataatat aaatttttat attattgatg gtatatctat 11641 tgcacaaaac ataggtttag gcggtcgaat taatatggta atgcaatctg cgttctttaa 11701 attggctaat gttatacctg taaatgaagc tgtagagctt ttaaagaatt cagttgaaaa 11761 aacctatggt aaaaaaggtg aaaagattgt agaaatgaac aaagcagcta ttgatgcagg 11821 tattgatgcg gtacacaagg ttaatattcc atcttcatgg aaaaatgttg aaactgaact 11881 agcttcaata aaaaaagaac ctgacttcat aaaaaaaatt caaagaccta tatctagaca 11941 tgaaggagat gaacttcctg ttagcgcttt taatggaatg gcagatggta ctttcccatt 12001 aggaaccact tcttacgaaa aacgtggtat tgctgttatg attccagaat ggcaaattga 12061 taaatgcatt caatgtaatc agtgctccta tatttgtcct cattcagtaa taagagctta 12121 cttattagat aaagatgaaa aagaaaaggc acctgataca tttgaaacaa aaaaagctgc 12181 tggaaaagga ttagaagatt tagaatatcg tattcaaata agttcattag attgtacagg 12241 atgtggtaat tgtgcagatg tttgtccagc accaggtaaa gccttaatta tgaaaaatgc 12301 ggaagaacaa attgaaatgc aatctgaaaa ctgggaattt ggtcttaata ttactactaa 12361 agagaactta atggatccaa aaacattaaa aggcagccag tttataagac cattactaga 12421 gtttcacgga gcttgtcctg gatgtggaga aactccatat attaaacttc taacacagct 12481 ctttggcgat agaatgatga ttgccaatgc tactggatgt tcctcaattt ggggaggtag 12541 tgctccatca gttccatata ctacaaattc acaaggcaaa ggcccttctt ggggtaattc 12601 tctatttgaa gataatgctg aatatggtta tggaatgtac cttgctgtaa aacaggttag 12661 agaaagatta gctaatttaa tgcaagaagc attaaatatg gaaattaacc agaatcttaa 12721 agagactttt aaacaatggt tggattcaat ggatgatggt gaaggctcaa aaattgctac 12781 agcgaaaata ctagaaacta tatcaaatga aaattatcaa aataatccta tcttaaatga 12841 aataatggag aaaaaagact atcttataaa aaaatctcac tggatgatag gtggagatgg 12901 ttgggcatat gatataggtt ttgggggctt agatcacgtg ttagcttctg gggatgatgt 12961 taatatattt gtaatggata cagagatcta ttctaatact ggtggtcaat gctcgaagtc 13021 tactcaaact ggtgctatag ctaaatttgc tgctgctggt aaaaaaatca agaaaaaaga 13081 tctggggctt atggcaatga gttacggata tgtatatgta gctcaaatag ctatgggcgc 13141 aaatatgaac catactatta aaacaattgc agaagcagaa gcatataaag gaccttcatt 13201 aataattgca tatgctcctt gtattagtca tggaataaaa acaggcatgg gaacaagtat 13261 agcagaagag aaaaaagcag tccaatcagg ttattggcat ctttatagat ataatccaat 13321 gttaaaagaa gaagataaga atccattcat cttagattca aaagaaccaa caaaaccttt 13381 taaagaattt attcaaggag aagttcgata ctcttctcta atgaatatat tccctgatat 13441 tgctgcaaaa atgtcagata tggctgaaca acatgctcgt gaaagatacg acaactatag 13501 gcatttagca gataaatagt ataaatgaaa aatagaaggg aacctataag tttcccttct 13561 atttttgatt agtatctacc cattctttgg catttctcac ttcaacttca cttgggatag 13621 atacattaca aataggtttc atactttcga tatttgccca cgcggcagta tcatgctttt 13681 ctataggcat tgccataaaa ctttccttat tattgttttt atccactact atcgcaccct 13741 tcaattatat ctttagttta ttttagtatt ccctaaatag atttacatta atctaaatct 13801 attatatatt ttaaaattta agtcttataa tatcattgtg aattccaaac caaattatat 13861 ggaaaatctt ctaatccata tccaattttt taaaaaatta acaatttatt tatttacctc 13921 actacttatt ttataatttg tatgcactca tacttccatc cgaaattcaa ccacatgact 13981 aaatttcttt ctaaggactg aataagttca gcgtgacttt tcattaacac ttatatcctt 14041 ttttcttata taacaaatag ttcaagagtt ttaaaacttt atctagtcat taaataatgc 14101 tacaattttg taaatataat ctatttcatt tctaagctgc ttatcttttt tctcctgaat 14161 taaccttatt acttctttag agttatctgt aataatacta tctacttgta actctataaa 14221 cttttccatt ttttctggat catttattgt ccaaacatga atactcttat tatttttatg 14281 cactttctca ataaattctg gcgttgcaac ggcttcttct acacttaaga agtccacatt 14341 caatgcagta acatctccca tacctgcaaa tacaatatat cccactttga ttctagaatt 14401 taaatttttt accttttgca ggacattata atctaatgac gtaactacac attgatctac 14461 aaaattattg tcctctataa gctttactac cctttcttct agcttttgat catgtccatt 14521 cagtttaatt tctatattca gctttatatt tttattggct gatttcataa tgctgtccaa 14581 ggttggaatt ttctcgccag caaacttagg tgaaaaatga cttcccacat ctaatttttc 14641 tacttcatca aaggtcaatt cccaaatatt tttgtttacc cctgtattgt tctttaaatt 14701 ggtatcgtgg gttaaaacca ccactccatc ctttgtttcc tgtacgtcta gctcactata 14761 atctgctttg ttgtcaatag cgcttaaaag tgaactcata gtattatctg gtgcattaac 14821 agcatctccc ctgtgagcgg taacagtaac caaattgtta ttagcatcga attcataagt 14881 aataaaataa atataggaaa gccatgtaaa tatcattatt cctagcacac taactgctat 14941 taattttttc ttatgtcgat taaataaatt ttgccagttt attttgcttg gtgttactgt 15001 aataatcata ggggttggtt ctacaccatc tttcattttg gcttgtctat agtacaattg 15061 agtaatcaac ataatagtaa gtggataaat aaataaagtg gctatcatga ctaatattcc 15121 acccattgtt gtaacaaagg aaagaatagt tacaaatagt ttcacatttc cttttagtac 15181 ttcatttaaa agcaccatac caattcccac taaaatcacc actactgcta caatagcaaa 15241 aatacttaag ttccagaaaa acatagtagt ccaaatttta agaaaagatc cttttacaag 15301 ctgtgaactc ctctttaatg cactaaaaaa atcctttcct tccaatacta caacgtgtaa 15361 tgaaaaaatc catctaatta aaaaatataa tagtataaaa aatacaattg catataaact 15421 tatccatttt ggtgttttgt atactacatc cataataaag gctggtattt caatcttcgg 15481 gagtaaactt gaagacaacc ccattgaact agctacaaaa ggaataataa aaagaacata 15541 tattacaatt ggaatagagc ctatttttat aatacctcta atacttttca ttgctgtaaa 15601 aaaagatgct tttgctgaaa tctttttatc aaagtatgtt tcttgtgata ttacaataag 15661 ccctgcatat tctataaaaa taaaaaggaa gctaagaatt cccaaaatta aaattgtcaa 15721 aattcctgga atacttccta taaaacttaa tatttggtta tttgccatca tcttcatccc 15781 tcttagatgc ataaaataat ttagcaccat tgtaataaat gggagaaata taaagcttgt 15841 caacactttg tatatcgatt caaatccaat atttataaaa aaactctttt taaaataggt 15901 gaaatttttt cttatcatct ttaatgtgga tatgggttta ttatgctttg tctccattct 15961 cgttgtcatt gatctgtccc ctttacattt tctttttcaa tttagctaca ataaataccg 16021 ctaaaattaa catacattat tatgtttttc ttatataata acaaccattt cattgttttt 16081 aaatatgttt tttataaata atatatattt cttgtggctt tataatatat ataagattat 16141 cttaagaagc actatacatc tttacatcta caccgtaaaa attttgtcta cccccccccc 16201 gatgttaaaa taactatctt aagaatcaaa aaattttata catcacttag aaattaatac 16261 taaattcctt ggtcttgata tagaaattaa aagtaaagaa aaaagtgctc catcctcaca 16321 agattagcac tttttcttgt taacatattt cattaaaatc aatagcccta aaccaaaata 16381 tatttcctat tgaactttat aacactatat ccttcctttc gaccacaact atactaagta 16441 ctaagaaaac agttatatat atacatgcag taagtgaaag cattagaata ttatgggcat 16501 ttaaattttg gacaatccta taagcataca ttcctccaga aaatgtataa tacattatat 16561 ttttcatctg agaatctatc atcatcataa taacatcaat tccaattccc aatcctatca 16621 ttgcaccact attattaata atcattgcaa taaaacttac aactgcgcta aaggataata 16681 aaggtaatat tatactagca taaactttta tattattaat aaacaaattt aagttatctc 16741 cattattccc caaaccaaaa gcgatagccc caacaatata acttccaaca aaacccacaa 16801 ttaaaaatat taacgcatat aaagctataa ataaaaattt tccaattata aaatcacttt 16861 tcttaatagg agtcattaat gaaaatttca tagttccatg aatataatct tctgttaatg 16921 tttcagctat tactaacacc ataaatattg gaaccattgg tttcattagc attccaaaaa 16981 gagtttcaat cacaaaatta tttgcattca ttccatcctg tggatgagat tttcccaaaa 17041 aagacataat aactatactt ataatcaaaa tccctgacaa tacataaaac tttttaccac 17101 taaacatttt tataatttca ttttttaaaa ccttaaacat attttttatc tccttccatt 17161 acattgaaaa aataatcctc taatgaagtt tcactgcaat gtagattttc aacattaaca 17221 tcattagaaa ttaaaagctt atttatattt tgaaaattac ccttttttat cttaattctt 17281 ataccacctt caaaacattg aatttttgag gaaatattca tattttttaa tagacgagag 17341 gttttctcct tttcttttgt ataaatttct attacttcct catctttatt taagagatcc 17401 tccacataac cttcaacctt aagactcccc ttttctataa ttcctattct attacatact 17461 gcttgaattt cacttaatat gtgtgatgat ataagaaatg ttacatgata tttttttgat 17521 aaatcctgta taagtcccct tatatccttt attccttgag ggtctaatcc attagttggc 17581 tcatctaata taactatatt gggattattt aaaaaagctc tcgcaattcc aagcctttgt 17641 ttcattccta gagaatattt agacacattt ttatgagcta catcagttaa tctcaccatt 17701 tggagtactt catctactct ttttttagga acaccatata gattagccat aagaataaga 17761 tttttatatc ctgatactcc tccataaaag cttggtgctt ctaccatagc tcctattttc 17821 tcaatagcct tttcactatc tttttctaca tcatatccat taataataac cttacctgaa 17881 gtgggtttta taagtcccat aatgctcttt atggttgtac tttttcctgc tccatttggt 17941 cctaaaaacc cataaacatc tccttcatat acagtaatat taagattatt tactgctttt 18001 ttatttttat attctttagt aagattatga gtacttaata aacatttttt tttcatatat 18061 aatcaactcc tcttaatatc tgttgcatat caacatacat tacttgtttt cacttgatga 18121 ttatatacta acaaagtttt atactctatt ttttcatttc ttcctaagtg aactttagct 18181 attcataaaa agctttattt ttacggttat gaaataaaac tttacggtta agaaaaaaga 18241 caccttcttt taaactgccc ccaaatgtta gtcaaaaaat ctaacacttg gagatgcaat 18301 tcaattaaga tgtcttttct atatatttta tccatatttc ctgaataaac atattctctt 18361 caatagatgt atttaaaaat acattttcat attttgtatc tataatgttt tttacagtat 18421 ataagcctaa gcctcttccc tttccttttg tagaaaatcc ttctttatat attttatgaa 18481 tcaacggctt gtctccaaaa aaattattat gtactgctat aaccacataa tcatttttat 18541 ttgcaacagc aatacatatc ttaggtgctt cacattctcc tgccgcttca atagaattat 18601 ccataagtat tcctattatt ctacacaaat ccattatatt catatgtata taatcaatat 18661 cttctacaat atgaacattt atgtctatat tcattgatga agcttttata agttttgttg 18721 ataatattgc ctttagtcct gaaactttaa tatatttaag tttatctata ttactattgc 18781 tccattttat atttttgtcc atatcaataa cgttattata aaaaaactca ctaagtcctt 18841 ctatatcttc attatctata tattccttaa gaggtgaaag catatttata taatcatgtt 18901 tgaatcttct tgtttcacta tacatctctt ctataatatg agcatattct tctatttcat 18961 ttttttcttc aatttctctt cttaattcaa ccctatattc aacactttta tcataataat 19021 aaactataat acttgtaagt ataattgata ttagtggcaa agcttctgga acaaaattgc 19081 ccataataaa atctatcttt tgattattta ttatagaggt gcatgtaaat atataaattg 19141 atgctatcat tacagttaat aatgtgctta tcattaaaat cattctagat cttatttggg 19201 atgatgaaat ttctttattt ctccttttct ttgattcagt tctttttatt attaaagatg 19261 cgaacactat aataggtaac attattataa attgaattaa tatataattt agtgatacat 19321 tatctaatct cgcaccccaa aatgcatata taaaagcata tattgtattt gccaaataca 19381 cactataaat actaatcaat gtttttattt tactttcatt ttcattattt cttaattgaa 19441 caatagttat aattgaaagt ataggaagta ttaaggtata tgaaccagtt gtaagcacca 19501 ttcctgttac aaatgaaata ataaaacatt ttttaatatt acttatattt attgtatttt 19561 taaatagaat atatatttaa atggaacata aaatactcat tagtacagat ggtattatca 19621 tatagcataa aatcatattt ataaacttag taaaagtcat accaagagtc ctctcatata 19681 tctaaatgaa acataacact cttcctcatt ttccattatt atcttaccat gcttcttgtc 19741 tatctcttca attttgtctt tgttcacaat atatgattta tgacatctat aaaaatttga 19801 aggaagtttt ttttgcatat cctttaagtt tgcatacaat tcaatctgtc tagtttcttc 19861 atgaatcctt attttatggg gagttcctgt agtttcaatg aatagaatat cactagatct 19921 aatgtttatt attcgtgtat ctgtatctat tgaaatgtat tcctcttcgt gttcttgttt 19981 atggtatcta ttataagcct ttataataca actatttact ctttctttaa aatcatcatc 20041 atccttaatc acataatcca tagcttctac tttatattta aatgctaaat aactcatttc 20101 taaatgagta gtagtaaata ttatatatcc taatggatct aattctctaa ttttttcccc 20161 cagctttata ccatttatat cattttttaa atctacatcc aaaagataaa taccagtgtt 20221 cctgttttct tttatataac ttattatttc ctcaggactt tctgtagcta gtacaacatc 20281 taaatctaat ttttctctta aaatagtatt ctctacaatt tgcttcagtt tatctctatg 20341 tacttcatta tcttcacaaa ttattactct taacattact tcttctcctt actttatata 20401 aaacttcttg gtattcatat ataaacatga aaaatactaa caacattttt tcccatgaaa 20461 gataatgttt tctttattta tattttagca aatttttata taaagtaaaa atttaaattt 20521 taatctagat atgtaaattt cacaaataaa aaacaatttg tttattaatg cgttaataaa 20581 cattattttt aatgttagaa aagagctaat aataatttat tgttcgactt ttaaaaaagc 20641 aaaataatgg gaaccaaatc ttttataatt acctatggtt tacctgtaaa cagtgaatcc 20701 aatgtatttg atcccaataa attattatta gtagtattta atccagtaac tattagtgat 20761 ttagttataa aaccaagatc caaggctaaa acttctcatg aattaatttt atattttgag 20821 gatagagcct tcttactctt tggaaatact gtccaataat cacatataat taaaatatat 20881 ttaaataaaa aaagattctc aaaatagatt ttattttgag aatctctttt atttctttac 20941 ttttcaatta ttcctccacc tattaataaa tcatctttat agaaaaccac agattgacct 21001 ttagttatag ccctttgttt ttcatgaaaa tttaccctta ctctaccatc ttctacagta 21061 gttattattg cctttgaagg aatttgagaa tatcttatct tagcctcaac ttccataggc 21121 tcttttaaag tatcaaaggg tatataattt gtatccttag ctataagttc tgtatttaaa 21181 agatcttcta aatctcctaa aactacttca tttctaaatg gatttatatc tactacatac 21241 ataggctttc caaaagctat acctaatcct tttctttgtc ctatggtata atatactatt 21301 cctttatgag tacctaatat attcccttgc ttatctacaa agttaccttc tctaacctta 21361 ttaggaaatc tatttttaat ataccttcca tgatcattat ctggtatgaa gcatatttct 21421 tcactatctt ttttattgtg aaccctaaga cctatttctt tagctatttc tctaatttta 21481 ctttttttat actgtccaca gggcatcaag gttctttcca attgaaattg tgtaagatta 21541 taaagagcgt aggtttgatc ttttttatcg tcttctgatt tctttattat atatctatca 21601 ttatgctttt ctatgatagc ataatgacct gtagccacat aatctatacc catagccatg 21661 gctttatcca gaaatgaaga aaactttata aatttattgc aggctatgca aggatttggt 21721 gttcttcctt ccatatattc atctacaaaa taatcaataa catttctttt aaaggcatct 21781 ttaaaattca taacataaaa aggaatgcct attttatttg ctaccctccg agcatccgct 21841 actgcactta aagagcaaca tccaccttct ttttctataa attcctcatc atcttgccaa 21901 atttgcatag ttactcctat aacctcataa ccctgctcct ttaaaagata cgctgctact 21961 gaactatcta ctcctccact cataccaact aaaactttct ttttcaaaat attcacctcc 22021 ctactaaatt attaaaatat ctatttactt attgtttttt ttaatacata ataaatagtt 22081 tttgctttac acaattattc ttatttacta caattttaaa attacttgct tttaggctta 22141 ttattaacca tattagtttt atttaagtca caacaatcta attttttatt ttcaaattct 22201 ttttcattag ccttttctaa tttttttatt aatcttttaa gtatgttcat attatcacct 22261 tacagattta tttttaatat aaatgaactt catcctaaga ctttttaaat tttatctgat 22321 acccatatat tgtgaatttt aatgtactag gaaaatttta aatttatttt actttcctaa 22381 ttattttctt taaactatat taaactagtt tttatttatt tcagtattta aactgcccat 22441 cttataccct tctgtatcta cagtaacata tttaaatcct aattttttaa atttttctga 22501 tactttatct agaatatcct cattgaacaa tttcactctt tcttttttag gcacttctat 22561 tctagccaaa tccccatgac ttcttactct tactgctcta aatcctaact ccatcatata 22621 tacttctgcc ttttctattc tagataaatc ttcttctttt atctcttgat tataaggaat 22681 tctagacaaa agacaggcat aggcaggttt attccaagtt tgtaacccta gctccttaga 22741 taaagttctt attttctctt tatttataga gttttctaag agaggacttt ttacatctaa 22801 ttcttttaat gctctcatac ctggtctata atcttttgta tcatccaaat tagttccatc 22861 tactatatat ttgacgccct tttcctttgc caattctttt attttactaa atacgccttt 22921 tttacatata tagcatctgt cttctggatt aaatcttatt tcttcaagca ttggaacttc 22981 taaaaaatat gattttattc ctatgttatt tgctaattct ttagcttctt ctatttccca 23041 ttttggaata taaggtgata ctactgtaat ggaaataaca ttatctccta aagcttcctt 23101 tgctgctttt aataataagg tactatctac acctccagaa aaagctactg ctacactacc 23161 taaatccttt aaatagttaa taagattatt atatttataa ttcattttat accatcccca 23221 tcttatattt ttgtcctatt attatgcttg ttaatatttc catctactat aaaaatcaag 23281 tatttgtata catttttgta agttaatata aaattaagct atatagatat atttatccga 23341 tgactacccg ctctaatact cccatcttct ttaaagtggg agtaaagagt gggtacatcc 23401 ctggataacg atttctaagc tttagaggga gtaaaaactc cccctgaagc caagaactct 23461 gtttataata atacaaatca tatcaattta aagattactt tactatttcc tttaatttt //